Deoxynivalenol induces apoptosis in chicken splenic lymphocytes via the reactive oxygen species-mediated mitochondrial pathway

被引：62

作者：

Ren, Zhihua ^{[1
,2
,3
]}

Wang, Yachao ^{[1
,2
,3
,4
]}

Deng, Huidan ^{[1
,2
,3
]}

Deng, Youtian ^{[1
,2
,3
]}

Deng, Junliang ^{[1
,2
,3
]}

Zuo, Zhicai ^{[1
,2
,3
]}

Wang, Ya ^{[1
,2
,3
]}

Peng, Xi ^{[1
,2
,3
]}

Cui, Hengmin ^{[1
,2
,3
]}

Shen, Liuhong ^{[1
,2
,3
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Yaan 625014, Sichuan, Peoples R China

[2] Sichuan Prov Key Lab Anim Dis & Human Hlth, Yaan 625014, Peoples R China

[3] Key Lab Environm Hazard & Human Hlth Sichuan Prov, Yaan 625014, Peoples R China

[4] Southwest Univ Sci & Technol, Sch Life Sci & Engn, Mianyang 621010, Peoples R China

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2015年 / 39卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Deoxynivalenol; Apoptosis; Mitochondria; Reactive oxygen species; IMMUNE-RESPONSES; FUSARIUM-MYCOTOXINS; BLOOD-LYMPHOCYTES; OXIDATIVE STRESS; P53; NETWORK; T-2; TOXIN; DEATH; PROLIFERATION; CELLS; DON;

D O I：

10.1016/j.etap.2014.11.028

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We investigated the immunotoxicity and cytotoxicity of deoxynivalenol (DON), a mycotoxin, and the mechanism by which it induces apoptosis. Chicken splenic lymphocytes treated with 0-50 mu g/mL DON for 48 h inhibited growth of splenic lymphocytes in a dose-dependent manner, as revealed by the Cell Counting Kit-8 (CCK-8) bioassay. Annexin V-fluorescein isothiocyanate staining indicated that the number of apoptotic and necrotic cells were significantly higher compared with the control (P < 0.01). DON treatment induced ROS accumulation, resulting in reduced mitochondrial transmembrane potential, as detected by flow cytometry and 2',7'-dichlorofluorescein acetate and rhodamine 123 labeling, respectively. Enzyme linked immunosorbent assays revealed that the concentrations of p53, Bax, Bak-1, and Caspase-3 increased with increasing DON concentration (P < 0.05 or P < 0.01), whereas the concentrations of Bcl-2 decreased (P < 0.01) compared with the control. These data suggest that DON induces apoptosis in splenic lymphocytes via a ROS-mediated mitochondrial pathway. (C) 2014 Published by Elsevier B.V.

引用

页码：339 / 346

页数：8

共 42 条

[1] Ingestion of low doses of deoxynivalenol does not affect hematological, biochemical, or immune responses of piglets [J].