Adaptive aberration correction in confocal scanning fluorescence microscopy

被引：3
|
作者：
Wang, Zhibin [1 ,3 ]
Shi, Guohua [1 ,2 ]
Zhang, Yudong [1 ,2 ]
机构：
[1] Chinese Acad Sci, Key Lab Adapt Opt, Chengdu 610209, Sichuan, Peoples R China
[2] Chinese Acad Sci, Inst Opt & Elect, Lab Adapt Opt, Chengdu 610209, Sichuan, Peoples R China
[3] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
来源：
CHINESE OPTICS LETTERS | 2014年 / 12卷
基金：
中国科学院西部之光基金; 美国国家科学基金会;
关键词：
D O I：
10.3788/COL201412.S11103
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Adaptive optics is implemented in a confocal scanning fluorescence microscopy using a wavefront sensorless correction scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. We demonstrate the idea of using phantom fluorescence samples experimentally. Enhanced imaging contrast and improved signal level are achieved.
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页数：3
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