"In vitro" 3D models of tumor-immune system interaction

被引：67

作者：

Hirt, Christian ^{[1
,2
]}

Papadimitropoulos, Adam ^{[2
,3
,4
]}

Mele, Valentina ^{[1
,2
]}

Muraro, Manuele G. ^{[1
,2
]}

Mengus, Chantal ^{[1
,2
]}

Iezzi, Giandomenica ^{[2
,5
]}

Terracciano, Luigi ^{[6
]}

Martin, Ivan ^{[2
,3
]}

Spagnoli, Giulio C. ^{[1
,2
]}

机构：

[1] Univ Basel, Univ Basel Hosp, Dept Surg, Oncol Lab, CH-4031 Basel, Switzerland

[2] Univ Basel, Univ Basel Hosp, Dept Biomed, CH-4031 Basel, Switzerland

[3] Univ Basel, Univ Basel Hosp, Dept Surg, Tissue Engn Lab, CH-4031 Basel, Switzerland

[4] Cellec Biotek, Basel, Switzerland

[5] Univ Basel, Univ Basel Hosp, Dept Surg, Canc Immunotherapy Lab, CH-4031 Basel, Switzerland

[6] Univ Basel, Inst Pathol, CH-4031 Basel, Switzerland

来源：

ADVANCED DRUG DELIVERY REVIEWS | 2014年 / 79-80卷

基金：

瑞士国家科学基金会;

关键词：

Cancer; Immune system; Three-dimensional cultures; Hypoxia; Perfusion bioreactors; EPITHELIAL-MESENCHYMAL TRANSITION; 3-DIMENSIONAL PERFUSION CULTURE; ANTI-EGFR ANTIBODY; T-CELLS; MELANOMA-CELLS; MULTICELLULAR TUMOR; LACTIC-ACID; TGF-BETA; INFILTRATING LYMPHOCYTES; ENGINEERING TUMORS;

D O I：

10.1016/j.addr.2014.05.003

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Interaction between cancer cells and immune system critically affects development, progression and treatment of human malignancies. Experimental animal models and conventional "in vitro" studies have provided a wealth of information on this interaction, currently used to develop immune-mediated therapies. Studies utilizing three-dimensional culture technologies have emphasized that tumor architecture dramatically influences cancer cell immune system interaction by steering cytokine production and regulating differentiation patterns of myeloid cells, and decreasing the sensitivity of tumor cells to lymphocyte effector functions. Hypoxia and increased production of lactic acid by tumor cells cultured in 3D architectures appear to be mechanistically involved. 3D culture systems could be further developed to (i) include additional cell partners potentially influencing cancer cell-immune system interaction, (ii) enable improved control of hypoxia, and (iii) allow the use of freshly derived clinical cancer specimens. Such advanced models will represent new tools for cancer immunobiology studies and for pre-clinical assessment of innovative treatments. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：145 / 154

页数：10

共 137 条

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