Uptake of 99mTcN-MIBI in tumor cells in vitro and the comparison of it with 99mTc-MIBI

In this study, the uptake of (TcN)-Tc-99m-MIBI and Tc-99m-MIBI were investigated by colorectal cancer cell line CL-187, lung cancer cell line L-973 and breast cancer cell line MCF-7 in vitro, respectively. The results of uptake kinetics shows that (TcN)-Tc-99m-MIBI has maximal accumulations between 20 and 40 min after incubation in all of these tumor cells, and the accumulation (D%) of (TcN)-Tc-99m-MIBI was found to be significantly higher than that of Tc-99m-MIBI, especially in colorectal cancer cell line CL-187. Uptake of (TcN)-Tc-99m-MIBI decreased in the order MCF-7-->CL-187-->L-973. The maximal accumulation occurring at 40 min after incubation of (TcN)-Tc-99m-MIBI in MCF-7 with the uptake rate about 30% per 10(5) cells. The influences of the culture conditions, such as cell concentration and incubation temperature, on the accumulation were also investigated, and the uptake of Tc-99m-complexes by cells were affected significantly by the culture conditions.