Effect of a Vietnamese Cinnamomum cassia essential oil and its major component trans-cinnamaldehyde on the cell viability, membrane integrity, membrane fluidity, and proton motive force of Listeria innocua

被引：50

作者：

Nga-Thi-Thanh Trinh ^{[1
]}

Dumas, Emilie ^{[1
]}

Mai Le Thanh ^{[2
]}

Degraeve, Pascal ^{[1
]}

Ben Amara, Chedia ^{[1
]}

Gharsallaoui, Adem ^{[1
]}

Oulahal, Nadia ^{[1
]}

机构：

[1] Univ Lyon 1, ISARA Lyon, Bioingn & Dynam Microbienne Interfaces Alimentair, BioDyMIA,EA 3733,IUT Lyon, F-01000 Bourg En Bresse, France

[2] Inst Polytech Hanoi, Ecole Biotechnol & Technol Alimentaire, Hanoi 10000, Vietnam

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2015年 / 61卷 / 04期

关键词：

Cinnamomum cassia essential oil; antibacterial activity; proton motive force; membrane fluidity; membrane integrity; ANTIBACTERIAL PROPERTIES; ANTIMICROBIAL ACTIVITIES; ESCHERICHIA-COLI; IN-VITRO; MONOCYTOGENES; PLANT; CONSTITUENTS; MECHANISMS; PATHOGENS; VIRULENCE;

D O I：

10.1139/cjm-2014-0481

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The antibacterial mechanism of a Cinnamomum cassia essential oil from Vietnam and of its main component (trans-cinnamaldehyde, 90% (m/m) of C. cassia essential oil) against a Listeria innocua strain was investigated to estimate their potential for food preservation. In the presence of C. cassia essential oil or trans-cinnamaldehyde at their minimal bactericidal concentration (2700 mu g.mL(-1)), L. innocua cells fluoresced green after staining with Syto9 (R) and propidium iodide, as observed by epifluorescence microscopy, suggesting that the perturbation of membrane did not cause large pore formation and cell lysis but may have introduced the presence of viable but nonculturable bacteria. Moreover, the fluidity, potential, and intracellular pH of the cytoplasmic membrane were perturbed in the presence of the essential oil or trans-cinnamaldehyde. However, these membrane perturbations were less severe in the presence of trans-cinnamaldehyde than in the presence of multicomponent C. cassia essential oil. This indicates that in addition to trans-cinnamaldehyde, other minor C. cassia essential oil components play a major role in its antibacterial activity against L. innocua cells.

引用

页码：263 / 271

页数：9

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