Detection of cytolethal distending toxin genes in strains of Campylobacter jejuni isolated from broiler carcasses

被引：10

作者：

Carvalho, A. F. ^{[1
]}

Silva, D. M. ^{[1
]}

Azevedo, S. S. ^{[2
]}

Piatti, R. M. ^{[1
]}

Genovez, M. E. ^{[1
]}

Scarcelli, E. ^{[1
]}

机构：

[1] Inst Biol, BR-04014002 Sao Paulo, Brazil

[2] Univ Fed Campina Grande, Patos de Minas, PB, Brazil

来源：

ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA | 2010年 / 62卷 / 05期

关键词：

broiler; Campylobacter jejuni; cytolethal distending toxin; PCR;

D O I：

10.1590/S0102-09352010000500006

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Eighty samples of refrigerated broiler thighs purchased in street markets and supermarkets in the city of Sao Paulo, SP, were analyzed. Thirteen Campylobacter spp. strains were isolated in 10 (12.5%) thighs, five of them from street market samples and other five from supermarkets. Eleven strains were identified as Campylobacter jejuni and two of them as Campylobacter coli. The 11 strains were confirmed to be C. jejuni using PCR for hippuricase (hip) gene. From these, multiplex-PCR showed that four (36.4%) strains presented the three genes (cdtA, cdtB, and cdtC) encoding cytolethal distending toxin: three strains from supermarket and one from street market samples. These results are important, because they demonstrate the presence of virulent C. jejuni strains in refrigerated broiler thigh samples, not only in the slaughterhouse but in the final point of the distribution chain, at the two most important food retail commercer.

引用

页码：1054 / 1061

页数：8

共 37 条

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