Determination of methyl order parameters using solid state NMR under off magic angle spinning

被引：10

作者：

Xue, Kai ^{[1
,2
]}

Mamone, Salvatore ^{[3
]}

Koch, Benita ^{[1
,2
]}

Sarkar, Riddhiman ^{[1
,2
]}

Reif, Bernd ^{[1
,2
]}

机构：

[1] Deutsch Forschungszentrum Gesundheit & Umwelt, Helmholtz Zentrum Munchen HMGU, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany

[2] Tech Univ Munich, Munich Ctr Integrated Prot Sci CIPS M, Dept Chem, Lichtenbergstr 4, D-85747 Garching, Germany

[3] Max Planck Inst Biophys Chem, Gottingen, Germany

来源：

JOURNAL OF BIOMOLECULAR NMR | 2019年 / 73卷 / 8-9期

关键词：

Solid-state NMR; Off magic angle spinning; Spin Echo; Microcrystalline proteins; Perdeuteration; NUCLEAR-MAGNETIC-RESONANCE; SIDE-CHAIN DYNAMICS; MODEL-FREE APPROACH; BACKBONE DYNAMICS; DIPOLAR COUPLINGS; STAPHYLOCOCCAL NUCLEASE; RELAXATION; PROTEIN; SPECTROSCOPY; SPECTRA;

D O I：

10.1007/s10858-019-00253-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantification of dipolar couplings in biological solids is important for the understanding of dynamic processes. Under Magic Angle Spinning (MAS), order parameters are normally obtained by recoupling of anisotropic interactions involving the application of radio frequency pulses. We have recently shown that amide backbone order parameters can be estimated accurately in a spin-echo experiment in case the rotor spinning angle is slightly mis-calibrated. In this work, we apply this method to determine methyl order parameters in a deuterated sample of the SH3 domain of chicken a-spectrin in which the methyl containing side chains valine and leucine are selectively protonated.

引用

页码：471 / 475

页数：5

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