Early life stress alters emotional learning in a sex- and age-dependent manner with no impact on emotional behaviors

Neonatal adversity can impact neurodevelopmental trajectories. This study examined the long-term effects of maternal deprivation on day 9 (DEP9), associated or not to a stressor (saline injection [SAL]), on contextual fear conditioning (Experiment 1) and emotional behaviors (Experiment 2) in Wistar rats. Whole litters were either assigned to DEP9 or control groups, and on day 10, half of the litters in each group received an SAL or not (NSAL). DEP9-SAL male adolescents showed the longest freezing time and DEP9 adult males froze more than females. Females exhibited less anxiety-like behavior than males; DEP9-SAL females spent more time in the open arms and DEP9 males visited less the extremity of the open arm in the elevated plus maze. Early life stress increased conditioned and innate fear in males, but not in females, indicating a clear sexual dimorphism in the response to potentially threatening stimuli.