Poly(lactide-co-glycolide)/Hydroxyapatite Porous Scaffold with Microchannels for Bone Regeneration

被引:21
作者
Zhang, Ning [1 ,2 ]
Wang, Yang [1 ]
Xu, Wenpeng [1 ]
Hu, Yong [1 ]
Ding, Jianxun [2 ]
机构
[1] Shandong Univ, Hosp 2, Dept Foot & Ankle Surg, Jinan 250033, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130021, Peoples R China
基金
中国国家自然科学基金;
关键词
poly(lactide-co-glycolide); hydroxyapatite; porous scaffold; microchannel; cell ingrowth; mass exchange; bone tissue engineering; IN-VITRO; IMMERSION PRECIPITATION; TISSUE; FABRICATION; MEMBRANES;
D O I
10.3390/polym8060218
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Mass transfer restrictions of scaffolds are currently hindering the development of three-dimensional (3D), clinically viable, and tissue-engineered constructs. For this situation, a 3D poly(lactide-co-glycolide)/hydroxyapatite porous scaffold, which was very favorable for the transfer of nutrients to and waste products from the cells in the pores, was developed in this study. The 3D scaffold had an innovative structure, including macropores with diameters of 300-450 m for cell ingrowth and microchannels with diameters of 2-4 m for nutrition and waste exchange. The mechanical strength in wet state was strong enough to offer structural support. The typical structure was more beneficial for the attachment, proliferation, and differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs). The alkaline phosphatase (ALP) activity and calcium (Ca) deposition were evaluated on the differentiation of rBMSCs, and the results indicated that the microchannel structure was very favorable for differentiating rBMSCs into maturing osteoblasts. For repairing rabbit radius defects in vivo, there was rapid healing in the defects treated with the 3D porous scaffold with microchannels, where the bridging by a large bony callus was observed at 12 weeks post-surgery. Based on the results, the 3D porous scaffold with microchannels was a promising candidate for bone defect repair.
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页数:11
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