Development and use of a vaccinia virus neutralization assay based on flow cytometric detection of green fluorescent protein

被引：82

作者：

Earl, PL ^{[1
]}

Americo, JL ^{[1
]}

Moss, B ^{[1
]}

机构：

[1] NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 19期

关键词：

D O I：

10.1128/JVI.77.19.10684-10688.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.

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页码：10684 / 10688

页数：5

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