Human Cytomegalovirus Infection Induces High Expression of Prolactin and Prolactin Receptors in Ovarian Cancer

被引：12

作者：

Rahbar, Afsar ^{[1
,2
]}

AlKharusi, Amira ^{[3
]}

Costa, Helena ^{[1
,2
]}

Pantalone, Mattia Russel ^{[1
,2
]}

Kostopoulou, Ourania N. ^{[1
,2
]}

Cui, Huanhuan L. ^{[1
,2
]}

Carlsson, Joseph ^{[4
,5
]}

Radestad, Angelique Floter ^{[6
,7
]}

Soderberg-Naucler, Cecilia ^{[1
,2
]}

Norstedt, Gunnar ^{[8
]}

机构：

[1] Karolinska Inst, Dept Med, Div Microbial Pathogenesis, BioClinicum, S-17164 Solna, Sweden

[2] Karolinska Univ Hosp, Div Neurosurg, S-17164 Stockholm, Sweden

[3] Sultan Qaboos Univ, Coll Med & Hlth Sci, Dept Physiol, Muscat 135, Oman

[4] Karolinska Univ Hosp, Div Pathol & Cytol, S-17177 Stockholm, Sweden

[5] Karolinska Inst, Dept Oncol & Pathol, BioClinicum, S-17164 Solna, Sweden

[6] Karolinska Inst, Dept Womens & Childrens Hlth, S-17177 Stockholm, Sweden

[7] Karolinska Univ Hosp, Div Obstet & Gynecol, S-17177 Stockholm, Sweden

[8] Sultan Qaboos Univ, Coll Med & Hlth Sci, Dept Biochem, Muscat 135, Oman

来源：

BIOLOGY-BASEL | 2020年 / 9卷 / 03期

基金：

瑞典研究理事会;

关键词：

prolactin; prolactin receptor; HCMV; ovarian cancer; NITRIC-OXIDE SYNTHASE; BREAST-CANCER; 5-LIPOXYGENASE EXPRESSION; GROWTH-FACTOR; DOUBLE-BLIND; GLIOBLASTOMA; SURVIVAL; INHIBITION; CYCLOOXYGENASE-2; REACTIVATION;

D O I：

10.3390/biology9030044

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

One of the potential biomarkers for ovarian cancer patients is high serum level of prolactin (PRL), which is a growth factor that may promote tumor cell growth. The prolactin receptor (PRLR) and human cytomegalovirus (HCMV) proteins are frequently detected in ovarian tumor tissue specimens, but the potential impact of HCMV infection on the PRL system have so far not been investigated. In this study, HCMV's effects on PRL and PRLR expression were assessed in infected ovarian cancer cells (SKOV3) by PCR and Western blot techniques. The levels of both PRL and PRLR transcripts as well as the corresponding proteins were highly increased in HCMV-infected SKOV3 cells. Tissue specimens obtained from 10 patients with ovarian cancer demonstrated high expression of PRLR, HCMV-IE, and pp65 proteins. Extensive expression of PRLR was detected in all examined ovarian tumor tissue specimens except for one from a patient who had focal expression of PRLR and this patient was HCMV-negative in her tumor. In conclusion, PRL and PRLR were induced to high levels in HCMV-infected ovarian cancer cells and PRLR expression was extensively detected in HCMV-infected ovarian tissue specimens. Highly induced PRL and PRLR by HCMV infection may be of relevance for the oncomodulatory role of this virus in ovarian cancer.

引用

页数：15