Development of a chemiluminescence enzyme immunoassay for the detection of aflatoxin M1

被引：0
|
作者：
Lang, Yifan [1 ]
Chen, Zhenzhen [1 ]
Zhu, Lixin [1 ]
Liu, Renrong [1 ]
Qiu, Xuemei [1 ]
Li, Kaihong [1 ]
Yang, Fanfan [1 ]
Hu, Na [1 ]
机构：
[1] Jiangxi Sci & Technol Normal Univ, Sch Life Sci, Nanchang 330013, Jiangxi, Peoples R China
来源：
PROCEEDINGS OF THE 2016 3RD INTERNATIONAL CONFERENCE ON MATERIALS ENGINEERING, MANUFACTURING TECHNOLOGY AND CONTROL | 2016年 / 67卷
关键词：
aflatoxin M-1; chemiluminescence enzyme immunoassay;
D O I：
暂无
中图分类号：
TP [自动化技术、计算机技术];
学科分类号：
0812 ;
摘要：
An indirect competitive chemiluminescence enzyme immunoassay (CLEIA) for detecting aflatoxin M-1 (AFM(1)) was developed. In the experimentation, antigen coated concentration, antibody dilution proportion, pH value and ionic concentration of the buffer were optimized to obtain the best reaction condition. Finally, the 50% inhibitory concentration (IC50) of the optimized CLEIA is 0.11 ng/mL for skimmed milk powder, and the recovery ranged from 77.98% to 104.39%.
引用
收藏
页码：106 / 109
页数：4
相关论文
共 50 条
  • [1] Development of a portable field immunoassay for the detection of aflatoxin M1 in milk
    Sibanda, L
    De Saeger, S
    Van Peteghem, C
    INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1999, 48 (03) : 203 - 209
  • [2] Development and validation of an ultrasensitive chemiluminescent enzyme immunoassay for aflatoxin M1 in milk
    Magliulo, M
    Mirasoli, M
    Simoni, P
    Lelli, R
    Portanti, O
    Roda, A
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (09) : 3300 - 3305
  • [3] Development of chemiluminescent enzyme immunoassay for the determination of aflatoxin M1 in milk products
    Li, Ping
    Zhang, Yan
    Lei, Hong-tao
    Wang, Hong
    Xu, Zhen-lin
    Shen, Yu-dong
    Sun, Yuan-ming
    Pang, Jie
    Yang, Jin-yi
    FOOD AND AGRICULTURAL IMMUNOLOGY, 2015, 26 (02) : 157 - 169
  • [4] Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk
    Vdovenko, Marina M.
    Lu, Chuan-Chen
    Yu, Feng-Yih
    Sakharov, Ivan Yu.
    FOOD CHEMISTRY, 2014, 158 : 310 - 314
  • [5] Development and application of solvent-free extraction for the detection of aflatoxin m1 in dairy products by enzyme immunoassay
    Anfossi, Laura
    Calderara, Marianna
    Baggiani, Claudio
    Giovannoli, Cristina
    Arletti, Enrico
    Giraudi, Gianfranco
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2008, 56 (06) : 1852 - 1857
  • [6] Development and validation of a lateral flow immunoassay for the detection of aflatoxin M1 in raw and commercialised milks
    Pimpitak, Umaporn
    Rengpipat, Sirirat
    Phutong, Songchan
    Buakeaw, Anumart
    Komolpis, Kittinan
    INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, 2020, 73 (04) : 695 - 705
  • [7] Optimization of a lateral flow immunoassay for the ultrasensitive detection of aflatoxin M1 in milk
    Anfossi, Laura
    Baggiani, Claudio
    Giovannoli, Cristina
    Biagioli, Flavia
    D'Arco, Gilda
    Giraudi, Gianfranco
    ANALYTICA CHIMICA ACTA, 2013, 772 : 75 - 80
  • [8] Development and Application of an Optical Biosensor Immunoassay for Aflatoxin M1 in Bovine Milk
    Indyk, Harvey E.
    Chetikam, Sowmya
    Gill, Brendon D.
    Wood, Jackie E.
    Woollard, David C.
    FOOD ANALYTICAL METHODS, 2019, 12 (11) : 2630 - 2637
  • [9] Development and Application of an Optical Biosensor Immunoassay for Aflatoxin M1 in Bovine Milk
    Harvey E. Indyk
    Sowmya Chetikam
    Brendon D. Gill
    Jackie E. Wood
    David C. Woollard
    Food Analytical Methods, 2019, 12 : 2630 - 2637
  • [10] Development of an Electrochemical Biosensor for the Detection of Aflatoxin M1 in Milk
    Paniel, Nathalie
    Radoi, Antonio
    Marty, Jean-Louis
    SENSORS, 2010, 10 (10): : 9439 - 9448
12345