Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation

被引：15

作者：

Dong, Yongzhe ^{[1
,2
]}

Ruan, Jingya ^{[1
,2
]}

Ding, Zhijuan ^{[2
]}

Zhao, Wei ^{[1
]}

Hao, Mimi ^{[2
]}

Zhang, Ying ^{[2
]}

Jiang, Hongyu ^{[2
]}

Zhang, Yi ^{[1
,2
]}

Wang, Tao ^{[1
,2
]}

机构：

[1] Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, 10 Poyanghu Rd, Tianjin 301617, Peoples R China

[2] Tianjin Univ Tradit Chinese Med, Inst TCM, 10 Poyanghu Rd, Tianjin 301617, Peoples R China

来源：

MOLECULES | 2020年 / 25卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Allium mongolicum Regel; flavonoids; phenolic acids; mouse isolated intestine tissue; qualitative analysis; GLYCOSIDES; EXTRACTS; ANTIOXIDANT;

D O I：

10.3390/molecules25030577

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To clarify whether flavonoids and phenols in Allium mongolicum Regel have the effect of improving gastrointestinal function and analyze its quality, this study was designed to isolate and identify them from the aerial parts of A. mongolicum by using various chromatographic and spectrophotometric methods, a bioassay on motility of mouse isolated intestine tissue, as well as qualitative analysis using liquid chromatography/mass spectrometry (LC-MS) analysis. As a result, 31 flavonoids and phenolic acids were obtained and identified, including six new flavonoid glycosides, mongoflavonosides A(1) (1), A(2) (2), A(3) (3), A(4) (4), B-1 (5), B-2 (6), and four new phenolic acid glycosides, mongophenosides A(1) (7), A(2) (8), A(3) (9), B (10). Among them, eleven flavonoids and three phenolic acids showed significant increase in the height of mouse small intestinal muscle. It was a first systematic bioactive constituents' study for A. mongolicum on gastrointestinal tract. Furthermore, according to the retention time (t(R)) and the exact mass-to-charge ratio (m/z), thirty-one compounds were unambiguously identified by comparing to the standard references by using LC-MS. Then, on the basis of generalized rules of MS/MS fragmentation pattern, chromatographic behaviors, as well as biosynthetic laws of the 31 isolates, five flavonoid glycosides and one phenolic acid glycoside were tentatively speculated. On the basis of the study, a fast analysis method for flavonoids and phenolic acids in A. mongolicum was established.

引用

页数：21