Placental-Derived Mesenchyme Influences Chorionic Gonadotropin and Progesterone Secretion of Human Embryonic Stem Cell-Derived Trophoblasts

被引：11

作者：

Giakoumopoulos, Maria ^{[1
,2
]}

Siegfried, Leah M. ^{[1
,2
]}

Dambaeva, Svetlana V. ^{[1
,3
]}

Garthwaite, Mark A. ^{[1
,2
]}

Glennon, M. Clay ^{[1
]}

Golos, Thaddeus G. ^{[1
,2
,3
]}

机构：

[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53715 USA

[2] Univ Wisconsin, Dept Obstet & Gynecol, Madison, WI 53715 USA

[3] Univ Wisconsin, Dept Comparat Biosci, Madison, WI 53715 USA

来源：

REPRODUCTIVE SCIENCES | 2010年 / 17卷 / 09期

关键词：

Embryonic stem cells; trophoblast; embryoid body; implantation; DIFFERENTIATION; LINES; VASCULOGENESIS; IMPLANTATION; DERIVATION;

D O I：

10.1177/1933719110371853

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Studies of early placental development in humans are difficult because of limitations on experimental material availability from the perimplantation period. We used a coculture system to determine the effects of various effector cell types on trophoblast differentiation. Enhanced green fluorescent protein (EGFP)-expressing H1 human embryonic stem cells were used in co-suspension with human term placental fibroblasts (TPFs) and dermal fibroblasts (CI2F) to form combination embryoid bodies (EBs), with the goal of recapitulating placental morphogenesis through incorporation of placental mesenchymal cells. Overall, the results demonstrated that when using mesenchymal cells for EB preparation from term placentas (TPF), combination EB-derived trophoblasts secrete higher levels of human chorionic gonadotropin (hCG) and progesterone compared to EBs made without effector cells, whereas there was no effect of dermal fibroblasts. This is due to the secretory activity of the EB-derived trophoblasts and not due to the number of differentiated trophoblasts per EB, demonstrating that nontrophoblast cells of the placenta can influence trophoblast endocrine activity.

引用

页码：798 / 808

页数：11