Novel Electrochemical Aptasensor Based on Ordered Mesoporous Carbon/2D Ti3C2 MXene as Nanocarrier for Simultaneous Detection of Aminoglycoside Antibiotics in Milk

被引:10
作者
Yue, Fengling [1 ]
Liu, Mengyue [1 ]
Bai, Mengyuan [1 ]
Hu, Mengjiao [1 ]
Li, Falan [1 ,2 ,3 ]
Guo, Yemin [1 ,2 ,3 ]
Vrublevsky, Igor [4 ]
Sun, Xia [1 ,2 ,3 ]
机构
[1] Shandong Univ Technol, Sch Agr Engn & Food Sci, Zibo 255049, Peoples R China
[2] Shandong Prov Engn Res Ctr Vegetable Safety & Qua, Zibo 255049, Peoples R China
[3] Zibo City Key Lab Agr Prod Safety Traceabil, Zibo 255049, Peoples R China
[4] Belarusian State Univ Informat & Radioelect, Dept Informat Secur, Minsk 220013, BELARUS
来源
BIOSENSORS-BASEL | 2022年 / 12卷 / 08期
基金
中国国家自然科学基金;
关键词
broad-spectrum aptamer; aminoglycoside antibiotics; Ti3C2; MXene; ordered mesoporous carbon; electrochemical aptasensor; RESIDUES;
D O I
10.3390/bios12080626
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Herein, a novel electrochemical aptasensor using a broad-spectrum aptamer as a biorecognition element was constructed based on a screen-printed carbon electrode (SPCE) for simultaneous detection of aminoglycoside antibiotics (AAs). The ordered mesoporous carbon (OMC) was firstly modified on 2D Ti3C2 MXene. The addition of OMC not only effectively improved the stability of the aptasensor, but also prevented the stacking of Ti3C2 sheets, which formed a good current passage for signal amplification. The prepared OMC@Ti3C2 MXene functioned as a nanocarrier to accommodate considerable aptamers. In the presence of AAs, the transport of electron charge on SPCE surface was influenced by the bio-chemical reactions of the aptamer and AAs, generating a significant decline in the differential pulse voltammetry (DPV) signals. The proposed aptasensor presented a wide linear range and the detection limit was 3.51 nM. Moreover, the aptasensor, with satisfactory stability, reproducibility and specificity, was successfully employed to detect the multi-residuals of AAs in milk. This work provided a novel strategy for monitoring AAs in milk.
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页数:14
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