Development of a pigment-based whole-cell biosensor for the analysis of environmental copper

被引：45

作者：

Chen, Pei-Hsuan ^{[1
]}

Lin, Chieh ^{[1
]}

Guo, Kai-Hong ^{[1
]}

Yeh, Yi-Chun ^{[1
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, 88,Sect 4,Tingzhou Rd, Taipei 11677, Taiwan

来源：

RSC ADVANCES | 2017年 / 7卷 / 47期

关键词：

METALLIDURANS STRAIN CH34; ESCHERICHIA-COLI; CUPRIAVIDUS-METALLIDURANS; RALSTONIA-METALLIDURANS; RESISTANCE DETERMINANT; KINASE CUSS; METALS; SENSOR; IDENTIFICATION; CHEMOSENSOR;

D O I：

10.1039/c7ra03778c

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Using engineered microorganisms to detect heavy metals in the environment has proven to be highly effective and robust. This paper reports on the development of a novel microbial sensor for the detection of copper ions. To develop this microbial sensor, we screened and characterized various biological parts, including promoters, output signals, and hosts. In addition, we used the plant pigment betaxanthin to output fluorescent signals in order to reduce the detection time. The resulting whole-cell biosensor presented a good sensitivity when detecting copper ions in environmental samples including freshwater pond and tap water.

引用

页码：29302 / 29305

页数：4

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