Antiresorptive Agents are More Effective in Preventing Titanium Particle-Induced Calvarial Osteolysis in Ovariectomized Mice Than Anabolic Agents in Short-Term Administration

被引：11

作者：

Fu, Guangtao ^{[1
]}

Li, Shixun ^{[1
]}

Ouyang, Nengtai ^{[2
]}

Wu, Junyan ^{[3
]}

Li, Changchuan ^{[1
]}

Liu, Wei ^{[1
]}

Qiu, Junxiong ^{[1
]}

Peng, Peng ^{[1
]}

Qin, Ling ^{[4
]}

Ding, Yue ^{[5
]}

机构：

[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Orthoped, Yuexiu Dist, Peoples R China

[2] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Cellular & Mol Diagnost Ctr, Yuexiu Dist, Peoples R China

[3] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pharmaceut, Guangzhou, Guangdong, Peoples R China

[4] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Musculoskeletal Res Lab, Hong Kong, Hong Kong, Peoples R China

[5] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Guangdong Prov Key Lab Malignant Tumor Epigenet &, Guangzhou, Guangdong, Peoples R China

来源：

ARTIFICIAL ORGANS | 2018年 / 42卷 / 09期

基金：

中国国家自然科学基金;

关键词：

Ovariectomy; Wear particle; Osteolysis; Anabolics; Antiresorptives; TOTAL HIP-ARTHROPLASTY; NITROGEN-CONTAINING BISPHOSPHONATE; NF-KAPPA-B; BONE LOSS; IN-VITRO; POSTMENOPAUSAL OSTEOPOROSIS; MOUSE MODEL; FOLLOW-UP; PARATHYROID-HORMONE; ZOLEDRONIC ACID;

D O I：

10.1111/aor.13271

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Aseptic loosening due to wear particle-induced osteolysis is the main cause of arthroplasty failure and the influence of postmenopausal osteoporosis and anti-osteoporosis treatment on Titanium (Ti) particle-induced osteolysis remains unclear. 66 C57BL/6J female mice were used in this study. Ovariectomy (OVX) was performed to induce osteopenia mice and confirmed by micro-CT. The Ti particle-induced mouse calvaria osteolysis model was established subsequently and both OVX and Sham-OVX mice were divided into four groups, respectively: Ti (-) group, Ti group, Ti + zoledronic acid (ZOL) group (50ug/kg, local administration, single dose) and Ti + teriparatide (TPTD) group (40ug/kg/d, subcutaneous injection*14d). Mice calvarias were collected for micro-CT and histomorphometric analysis 2 weeks after particle induction. 8 weeks after bilateral OVX, significantly reduced BMD and microstructure parameters in both proximal tibia and calvaria were observed in OVX mice when comparing with Sham-OVX mice. OVX mice in Ti group had not only markly decreased BMD and BV/TV, but also significantly increased total porosity, eroded surface area and osteoclast numbers when comparing with Sham-OVX mice. Shown by Two-way ANOVA analysis, the interaction terms between OVX and Ti implantation on micro-CT and histomorphometry parameters didn't reach significant difference. As illustrated by micro-CT and histological analysis, ZOL treatment markedly inhibited Ti particle-induced osteolysis in OVX mice and Sham-OVX mice, and there were significant differences when comparing to both Ti and Ti+TPTD group. The combination of osteoporosis and Ti particle implantation result in aggravated bone resorption, accompanied with increased osteoclasts and excessive inflammation response. ZOL was more effective in preventing Ti particle-induced osteolysis in both OVX mice and Sham-OVX mice than TPTD in short-term administration. ZOL exert the protective effects on Ti particle-induced bone loss via the suppression of osteoclasts.

引用

页码：E259 / E271

页数：13