Isolation and inhibitory effect of anti-Vibrio substances from Pseudoalteromonas sp A1-J11 isolated from the coastal sea water of Kagoshima Bay

被引：16

作者：

del Castillo, Carmelo S. ^{[2
]}

Wahid, Md Iftekharul ^{[2
]}

Yoshikawa, Takeshi ^{[1
]}

Sakata, Taizo ^{[1
]}

机构：

[1] Kagoshima Univ, Fac Fisheries, Microbiol Lab, Kagoshima 8900056, Japan

[2] Kagoshima Univ, United Grad Sch Agr Sci, Kagoshima 8900065, Japan

来源：

FISHERIES SCIENCE | 2008年 / 74卷 / 01期

关键词：

anti-Vibrio substance; high-pressure liquid chromatography; Pseudoalteromonas; ultraviolet absorption spectrum;

D O I：

10.1111/j.1444-2906.2007.01507.x

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Among several marine antagonistic bacteria isolated from the sea water of Kagoshima Bay, Pseudoalteromonas sp. A1-J11 was found to produce anti-Vibrio substances. The anti-Vibrio substances were extracted from the culture supernatant of the strain with chloroform and isolated using reverse-phase (Cosmosil 75C(18)-OPN) column chromatography followed by high-pressure liquid chromatography (Mightysil RP-18 GP Aqua). Purified substances, designated as AVS-03a, c and d showed similar ultraviolet absorption spectra with lambda(max) at 215, 235, 315 and 327 nm in methanol. AVS-03d, the major anti-Vibrio substance, was thermostable up to 100 degrees C and pH stable over a pH range higher than 4.0, and also showed strong inhibitory activities, specifically against Vibrio harveyi strains.

引用

页码：174 / 179

页数：6

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