Optimum Extraction of Polysaccharides From Fruiting Body of Catathelasma ventricosum Using Response Surface Methodology

Response Surface Methodology (RSM) is employed to optimize the polysaccharides extraction technique of Catathelasma ventricosum. This experiment adopts extraction temperature, extraction time and feed liquid ratio as the influencing factors. The polysaccharides extraction amount of Catathelasma ventricosum and their biological activity are adopted as the response value. According to the combination test design of Box-Benhnken, RSM featuring three factors and three surfaces is adopted to obtain a ternary quadratic linear regression equation. Under the prerequisite of maximizing the biological activity, the optimal extraction conditions after the fitting analysis of the equation are obtained: extraction temperature: 85 degrees C; extraction time: 3h; ratio of material to liquid: 0.05. Under these conditions, the extraction of polysaccharides is about 7.95g. Besides, results suggest that the actually-measured value stays extremely close to the predicted value. Ultimately proved using RSM is accurate and effective to optimize polysaccharides extraction condition of Catathelasma ventricosum.