Effect of gene knockouts of L-tryptophan uptake system on the production of L-tryptophan in Escherichia coli

被引：21

作者：

Zhao, Zhijun ^{[1
,2
,3
]}

Chen, Sheng ^{[1
,2
,3
]}

Wu, Dan ^{[1
,2
,3
]}

Wu, Jing ^{[1
,2
,3
]}

Chen, Jian ^{[1
,2
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China

[2] Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

[3] Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

来源：

PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 02期

基金：

中国国家自然科学基金;

关键词：

L-Tryptophan; Uptake system; Gene knockout; Batch fermentation; Escherichia coli; CATABOLITE REPRESSION; AMINO-ACIDS; TRANSPORT; OPERON; HYPERPRODUCTION; OVERPRODUCTION; FERMENTATION; EXPRESSION; THREONINE; MUTANTS;

D O I：

10.1016/j.procbio.2011.11.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uptake of L-tryptophan in Escherichia coli was carried out by three distinct permeases, Mtr. TnaB, and AroP, respectively. In the present study, the three genes of L-tryptophan uptake system were knocked out from an L-tryptophan-producing strain of E. coli, respectively. The knockout mutants all showed lower L-tryptophan uptake activities and higher L-tryptophan production than their parent. Among the three genes, the knockout of mtr was most critical for both L-tryptophan uptake and L-tryptophan production. The uptake activity of L-tryptophan of the mtr mutant was 1.5 nmol min(-1) (mg dry weight)(-1), which was decreased by 48% when compared to that of the parent; the production of L-tryptophan of the mtr mutant was 14.7 g/l, which was increased by 34% when compared to that of the parent. Furthermore, the physiological and fermentation characteristics caused by gene knockouts were also analyzed. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：340 / 344

页数：5

共 31 条

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