Human lens lipids differ markedly from those of commonly used experimental animals

被引:119
作者
Deeley, Jane M. [2 ]
Mitchell, Todd W. [3 ]
Wei, Xiaojia [2 ]
Korth, John [2 ]
Nealon, Jessica R. [2 ]
Blanksby, Stephen J. [1 ,2 ]
Truscott, Roger J. W. [1 ]
机构
[1] Univ Sydney, Save Sight Inst, Sydney, NSW 2001, Australia
[2] Univ Wollongong, Sch Chem, Wollongong, NSW 2522, Australia
[3] Univ Wollongong, Sch Hlth Sci, Wollongong, NSW 2522, Australia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 2008年 / 1781卷 / 6-7期
基金
澳大利亚研究理事会;
关键词
cataract; mass spectrometry; electrospray ionisation; fatty acid; phospholipid; cholesterol; ether lipid;
D O I
10.1016/j.bbalip.2008.04.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electrospray ionisation tandem mass spectrometry has allowed the unambiguous identification and quantification of individual lens phospholipids in human and six animal models. Using this approach ca. 100 unique phospholipids have been characterised. Parallel analysis of the same lens extracts by a novel direct-insertion electron-ionization technique found the cholesterol content of human lenses to be significantly higher (ca. 6 times) than lenses from the other animals. The most abundant phospholipids in all the lenses examined were choline-containing phospholipids. In rat, mouse, sheep, cow, pig and chicken, these were present largely as phosphatidylcholines, in contrast 66% of the total phospholipid in Homo sapiens was sphingomyelin, with the most abundant being dihydrosphingomyelins, in particular SM(d18:0/16:0) and SM(d18:0/24:1). The abundant glycerophospholipids within human lenses were found to be predominantly phosphatidylethanolamines and phosphatidylserines with surprisingly high concentrations of ether-linked alkyl chains identified in both classes. This study is the first to identify the phospholipid class (head-group) and assign the constituent fatty acid(s) for each lipid molecule and to quantify individual lens phospholipids using internal standards. These data clearly indicate marked differences in the membrane lipid composition of the human lens compared to commonly used animal models and thus predict a significant variation in the membrane properties of human lens fibre cells compared to those of other animals. (c) 2008 Elsevier B.V. All rights reserved.
引用
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页码:288 / 298
页数:11
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