The yeast cell wall protein Pry3 inhibits mating through highly conserved residues within the CAP domain

被引:2
作者
Cottier, Stephanie [1 ]
Darwiche, Rabih [1 ,2 ]
Meyenhofer, Felix [1 ]
Debelyy, Mykhaylo O. [1 ]
Schneiter, Roger [1 ]
机构
[1] Univ Fribourg, Dept Biol, Chemin Musee 10, CH-1700 Fribourg, Switzerland
[2] Harvard Univ, Harvard Med Sch, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
来源
BIOLOGY OPEN | 2020年 / 9卷 / 06期
关键词
CAP protein family; Fatty acids; Sterols; GPI-anchored proteins; Mating; Cell wall; Saccharomyces cerevisiae; PATHOGENESIS-RELATED PROTEIN; SACCHAROMYCES-CEREVISIAE; SCP/TAPS PROTEIN; SUPERFAMILY; ACID; IDENTIFICATION; FUSION; KEY; LOCALIZATION; HOMOLOGY;
D O I
10.1242/bio.053470
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Members of the CAP/SCP/TAPS superfamily have been implicated in many different physiological processes, including pathogen defense, sperm maturation and fertilization. The mode of action of this class of proteins, however, remains poorly understood. The genome of Saccharomyces cerevisiae encodes three CAP superfamily members, Pry1-3. We have previously shown that Pry1 function is required for the secretion of sterols and fatty acids. Here, we analyze the function of Pry3, a GPI-anchored cell wall protein. Overexpression of Pry3 results in strong reduction of mating efficiency, providing for a cell-based readout for CAP protein function. Mating inhibition is a conserved function of the CAP domain and depends on highly conserved surface exposed residues that formpart of a putative catalytic metal-ion binding site. Pry3 displays polarized cell surface localization adjacent to bud scars, but is absent from mating projections. When overexpressed, however, the protein leaks onto mating projections, suggesting that mating inhibition is due to mislocalization of the protein. Trapping of the CAP domain within the cell wall through a GPI-anchored nanobody results in a dose-dependent inhibition of mating, suggesting that a membrane proximal CAP domain inhibits a key step in the mating reaction, which is possibly related to the function of CAP domain proteins in mammalian fertilization. This article has an associated First Person interview with the first author of the paper.
引用
收藏
页数:16
相关论文
共 61 条
  • [1] Tracing the Evolutionary History of the CAP Superfamily of Proteins Using Amino Acid Sequence Homology and Conservation of Splice Sites
    Abraham, Anup
    Chandler, Douglas E.
    [J]. JOURNAL OF MOLECULAR EVOLUTION, 2017, 85 (3-4) : 137 - 157
  • [2] Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating
    Aguilar, Pablo S.
    Heiman, Maxwell G.
    Walther, Tobias C.
    Engel, Alex
    Schwudke, Dominik
    Gushwa, Nathan
    Kurzchalia, Teymuras
    Walter, Peter
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (09) : 4170 - 4175
  • [3] ALI SA, 1995, BIOTECHNIQUES, V18, P746
  • [4] ConSurf 2016: an improved methodology to estimate and visualize evolutionary conservation in macromolecules
    Ashkenazy, Haim
    Abadi, Shiran
    Martz, Eric
    Chay, Ofer
    Mayrose, Itay
    Pupko, Tal
    Ben-Tal, Nir
    [J]. NUCLEIC ACIDS RESEARCH, 2016, 44 (W1) : W344 - W350
  • [5] Role of the transcription activator Ste12p as a repressor of PRY3 expression
    Bickel, Kellie S.
    Morris, David R.
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 2006, 26 (21) : 7901 - 7912
  • [6] BOEKE JD, 1987, METHOD ENZYMOL, V154, P164
  • [7] Breeden LL, 1997, METHOD ENZYMOL, V283, P332
  • [8] Emerging Insights into the Functions of Pathogenesis-Related Protein 1
    Breen, Susan
    Williams, Simon J.
    Outram, Megan
    Kobe, Bostjan
    Solomon, Peter S.
    [J]. TRENDS IN PLANT SCIENCE, 2017, 22 (10) : 871 - 879
  • [9] ALLURIN, AN AMPHIBIAN SPERM CHEMOATTRACTANT HAVING IMPLICATIONS FOR MAMMALIAN SPERM PHYSIOLOGY
    Burnett, Lindsey A.
    Washburn, Catherine A.
    Sugiyama, Hitoshi
    Xiang, Xueyu
    Olson, John H.
    Al-Anzi, Bader
    Bieber, Allan L.
    Chandler, Douglas E.
    [J]. INTERNATIONAL REVIEW OF CELL AND MOLECULAR BIOLOGY, VOL 295, 2012, 295 : 1 - 61
  • [10] A portrait of the "SCP/TAPS" proteins of eukaryotes - Developing a framework for fundamental research and biotechnological outcomes
    Cantacessi, C.
    Campbell, B. E.
    Visser, A.
    Geldhof, P.
    Nolan, M. J.
    Nisbet, A. J.
    Matthews, J. B.
    Loukas, A.
    Hofmann, A.
    Otranto, D.
    Sternberg, P. W.
    Gasser, R. B.
    [J]. BIOTECHNOLOGY ADVANCES, 2009, 27 (04) : 376 - 388