Optimization of SPME-Arrow-GC/MS Method for Determination of Free and Bound Volatile Organic Compounds from Grape Skins

被引:19
作者
Sikuten, Iva [1 ,2 ]
Stambuk, Petra [1 ,2 ]
Karoglan Kontic, Jasminka [1 ,2 ]
Maletic, Edi [1 ,2 ]
Tomaz, Ivana [1 ,2 ]
Preiner, Darko [1 ,2 ]
机构
[1] Univ Zagreb, Fac Agr, Dept Viticulture & Enol, Zagreb 10000, Croatia
[2] Univ Zagreb, Fac Agr, Ctr Excellence Biodivers & Mol Plant Breeding, Zagreb 10000, Croatia
来源
MOLECULES | 2021年 / 26卷 / 23期
关键词
SPME-Arrow; grape skins; volatile organic compounds; SOLID-PHASE MICROEXTRACTION; VITIS-VINIFERA L; CHROMATOGRAPHY-MASS SPECTROMETRY; CABERNET-SAUVIGNON GRAPES; WASTE-WATER; AROMA; WINE; EXTRACTION; AMINES;
D O I
10.3390/molecules26237409
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
(1) Background: Solid phase microextraction (SPME)-Arrow is a new extraction technology recently employed in the analysis of volatiles in food materials. Grape volatile organic compounds (VOC) have a crucial role in the winemaking industry due to their sensory characteristics of wine.; (2) Methods: Box-Behnken experimental design and response surface methodology were used to optimise SPME-Arrow conditions (extraction temperature, incubation time, exposure time, desorption time). Analyzed VOCs were free VOCs directly from grape skins and bound VOCs released from grape skins by acid hydrolysis.; (3) Results: The most significant factors were extraction temperature and exposure time for both free and bound VOCs. For both factors, an increase in their values positively affected the extraction efficiency for almost all classes of VOCs. For free VOCs, the optimum extraction conditions are: extraction temperature 60 degrees C, incubation time 20 min, exposure time 49 min, and desorption time 7 min, while for the bound VOCs are: extraction temperature 60 degrees C, incubation time 20 min, exposure time 60 min, desorption time 7 min.; (4) Conclusions: Application of the optimized method provides a powerful tool in the analysis of major classes of volatile organic compounds from grape skins, which can be applied to a large number of samples.
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页数:16
相关论文
共 41 条
[1]   Volatile composition of Merlot red wine and its contribution to the aroma: optimization and validation of analytical method [J].
Arcari, Stefany Grutzmann ;
Caliari, Vinicius ;
Sganzerla, Marla ;
Godoy, Helena Teixeira .
TALANTA, 2017, 174 :752-766
[2]   Retention Indices for Frequently Reported Compounds of Plant Essential Oils [J].
Babushok, V. I. ;
Linstrom, P. J. ;
Zenkevich, I. G. .
JOURNAL OF PHYSICAL AND CHEMICAL REFERENCE DATA, 2011, 40 (04)
[3]   Retention Indices for Most Frequently Reported Essential Oil Compounds in GC [J].
Babushok, Valeri I. ;
Zenkevich, Igor G. .
CHROMATOGRAPHIA, 2009, 69 (3-4) :257-269
[4]   Biogeneration of C13-norisoprenoid compounds:: experiments supportive for an apo-carotenoid pathway in grapevines [J].
Baumes, R ;
Wirth, J ;
Bureau, S ;
Gunata, Y ;
Razungles, A .
ANALYTICA CHIMICA ACTA, 2002, 458 (01) :3-14
[5]   The present and future of the international wine industry [J].
Bisson, LF ;
Waterhouse, AL ;
Ebeler, SE ;
Walker, MA ;
Lapsley, JT .
NATURE, 2002, 418 (6898) :696-699
[6]   Headspace solid-phase microextraction-gas chromatography-mass spectrometry for profiling free volatile compounds in Cabernet Sauvignon grapes and wines [J].
Canuti, Valentina ;
Conversano, Michael ;
Calzi, Marco Li ;
Heymann, Hildegarde ;
Matthews, Mark A. ;
Ebeler, Susan E. .
JOURNAL OF CHROMATOGRAPHY A, 2009, 1216 (15) :3012-3022
[7]   Screening of tropical fruit volatile compounds using solid-phase microextraction (SPME) fibers and internally cooled SPME fiber [J].
Carasek, Eduardo ;
Pawliszyn, Janusz .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2006, 54 (23) :8688-8696
[8]   Analysis of Volatile Compounds in Soju, a Korean Distilled Spirit, by SPME-Arrow-GC/MS [J].
Cha, Jiyoon ;
Chin, Young-Wook ;
Lee, Jun-Young ;
Kim, Tae-Wan ;
Jang, Hae Won .
FOODS, 2020, 9 (10)
[9]  
D'Onofrio C., 2013, Sweet, reinforced and fortified wines: grape biochemistry, technology and vinification, P91
[10]   Wine Aroma Compounds in Grapes: A Critical Review [J].
Gonzalez-Barreiro, Carmen ;
Rial-Otero, Raquel ;
Cancho-Grande, Beatriz ;
Simal-Gandara, Jesus .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 2015, 55 (02) :202-218