Fabricating Gradient Hydrogel Scaffolds for 3D Cell Culture

被引：0

作者：

Chatterjee, Kaushik ^{[1
,2
]}

Young, Marian F. ^{[2
]}

Simon, Carl G., Jr. ^{[1
]}

机构：

[1] NIST, Div Polymers, Gaithersburg, MD 20899 USA

[2] Natl Inst Dent & Craniofacial Res, Craniofacial & Skeletal Dis Branch, NIH, Bethesda, MD USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2011年 / 14卷 / 04期

关键词：

Combinatorial methods; high-throughput screening; hydrogels; elastic modulus; gradients; osteoblast; tissue engineering; BIOMATERIALS;

D O I：

10.2174/138620711795222455

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.

引用

页码：227 / 236

页数：10

共 24 条

[11] Soft biological materials and their impact on cell function [J].

Levental, Ilya ;

Georges, Penelope C. ;

Janmey, Paul A. .

SOFT MATTER, 2007, 3 (03) :299-306

[12] Osteoblast response to dimethacrylate composites varying in composition, conversion and roughness using a combinatorial approach [J].

Lin, Nancy J. ;

Lin-Gibson, Sheng .

BIOMATERIALS, 2009, 30 (27) :4480-4487

[13] Structure-property relationships of photopolymerizable poly(ethylene glycol) dimethacrylate hydrogels [J].

Lin-Gibson, S ;

Jones, RL ;

Washburn, NR ;

Horkay, F .

MACROMOLECULES, 2005, 38 (07) :2897-2902

[14] Synthesis and characterization of PEG dimethacrylates and their hydrogels [J].

Lin-Gibson, S ;

Bencherif, S ;

Cooper, JA ;

Wetzel, SJ ;

Antonucci, JM ;

Vogel, BM ;

Horkay, F ;

Washburn, NR .

BIOMACROMOLECULES, 2004, 5 (04) :1280-1287

[15] Great expectations: Private sector activity in tissue engineering, regenerative medicine, and stem cell therapeutics [J].

Lysaght, Michael J. ;

Jaklenec, Ana ;

Deweerd, Elizabeth .

TISSUE ENGINEERING PART A, 2008, 14 (02) :305-U57

[16] Combinatorial characterization of cell interactions with polymer surfaces [J].

Meredith, JC ;

Sormana, JL ;

Keselowsky, BG ;

García, AJ ;

Tona, A ;

Karim, A ;

Amis, EJ .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 66A (03) :483-490

[17]

Mitragotri S, 2009, NAT MATER, V8, P15, DOI [10.1038/NMAT2344, 10.1038/nmat2344]

[18] The effects of matrix stiffness and RhoA on the phenotypic plasticity of smooth muscle cells in a 3-D biosynthetic hydrogel system [J].

Peyton, Shelly R. ;

Kim, Peter D. ;

Ghajar, Cyrus M. ;

Seliktar, Dror ;

Putnam, Andrew J. .

BIOMATERIALS, 2008, 29 (17) :2597-2607

[19] Substrate Modulus Directs Neural Stem Cell Behavior [J].

Saha, Krishanu ;

Keung, Albert J. ;

Irwin, Elizabeth F. ;

Li, Yang ;

Little, Lauren ;

Schaffer, David V. ;

Healy, Kevin E. .

BIOPHYSICAL JOURNAL, 2008, 95 (09) :4426-4438

[20] Cell Interactions with Biomaterials Gradients and Arrays [J].

Simon, Carl G. ;

Yang, Yanyin ;

Thomas, Vinoy ;

Dorsey, Shauna M. ;

Morgan, Abby W. .

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING, 2009, 12 (06) :544-553

← 1 2 3 →