N,N-Dimethyl chitosan/heparin polyelectrolyte complex vehicle for efficient heparin delivery

被引：42

作者：

Bueno, Pedro V. A. ^{[1
]}

Souza, Paulo R. ^{[1
]}

Follmann, Heveline D. M. ^{[1
]}

Pereira, Antonio G. B. ^{[1
,2
]}

Martins, Alessandro F. ^{[1
,2
]}

Rubira, Adley F. ^{[1
]}

Muniz, Edvani C. ^{[1
]}

机构：

[1] Univ Estadual Maringa, Dept Quim, GMPC, BR-87020900 Maringa, Parana, Brazil

[2] Univ Tecnol Fed Parana UTFPR, BR-85660000 Dois Vizinhos, Parana, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 75卷

关键词：

N; N-Dimethyl chitosan; Heparin; Polyelectrolyte complex; ANTIBACTERIAL MULTILAYER FILMS; INDUCED THROMBOCYTOPENIA; DRUG-DELIVERY; N; N-TRIMETHYL CHITOSAN; SILVER NANOPARTICLES; ABSORPTION ENHANCER; CONTROLLED-RELEASE; DERIVATIVES; ANTIADHESIVE; CYTOTOXICITY;

D O I：

10.1016/j.ijbiomac.2015.01.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Polysaccharide-based device for oral delivery of heparin (HP) was successfully prepared. Previously synthesized N,N-dimethyl chitosan (DMC) (86% dimethylated by H-1 NMR spectroscopy) was complexed with HP by mixing HP and DMC aqueous solutions (both at pH 3.0). The polyelectrolyte complex (PEC) obtention was confirmed by infrared spectroscopy (FTIR), thermogravimetric analysis (TGA/DTG) and wide:angle X-ray scattering (WAXS). In vitro controlled release assays of HP from PEC were investigated in the simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). The PEC efficiently protected the HP in SGF condition in which HP is degraded. On the other hand, in SIF PEC promoted the releasing of 80 +/- 1.5% of loaded HP. The promissory results indicated that the PEC based on DMC/HP presented potential as drug-carrier matrix, since biological activity of HP was improved at pH close to physiological condition. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：186 / 191

页数：6

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