Staurosporine Induces the Generation of Polyploid Giant Cancer Cells in Non-Small-Cell Lung Carcinoma A549 Cells

被引：19

作者：

Glassmann, Alexander ^{[1
]}

Garcia, Carmen Carrillo ^{[2
]}

Janzen, Viktor ^{[2
]}

Kraus, Dominik ^{[3
]}

Veit, Nadine ^{[4
]}

Winter, Jochen ^{[5
]}

Probstmeier, Rainer ^{[4
]}

机构：

[1] Life Sci Inkubator, Bonn, Germany

[2] Univ Bonn, Dept Internal Med 3, Div Hematol & Oncol, Bonn, Germany

[3] Univ Bonn, Dept Prosthodont Preclin Educ & Mat Sci, Bonn, Germany

[4] Univ Bonn, Dept Nucl Med, Neuro & Tumor Cell Biol Grp, Bonn, Germany

[5] Univ Bonn, Dept Periodontol Operat & Prevent Dent, Oral Cell Biol Grp, Bonn, Germany

来源：

ANALYTICAL CELLULAR PATHOLOGY | 2018年 / 2018卷

关键词：

ANALOGS; SENESCENCE; EXPRESSION; TUMORS; CYCLE;

D O I：

10.1155/2018/1754085

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Cultivation of A549 non-small-cell lung carcinoma (NSCLC) cells in the presence of staurosporine (SSP) leads to a reduction or a lack of proliferation in a concentration-dependent manner. This inhibition of proliferation is accompanied by the generation of polyploid giant cancer cells (PGCCs) that are characterized by cell flattening, increased cell size, polyploidy, and polynucleation as determined by crystal violet staining, BrdU and DiI labelling, and flow cytometry as well as video time-lapse analysis. Continuous SSP treatment of A549 cells can preserve PGCCs for at least two months in a resting state. Upon removal of SSP, A549 PGCCs restart to divide and exhibit a proliferation pattern and cellular morphology indistinguishable from cells where PGCCs originally derived from. Thus, SSP-treated A549 cells represent a simple and reliable experimental model for the reversible generation of PGCCs and their subsequent experimental analysis.

引用

页数：7

共 26 条

[21] Effect of staurosporine on the mobility and invasiveness of lung adenocarcinoma A549 cells: an in vitro study [J].