Determination of the survival of yeast and bacteria under the influence of gamma or UV radiation in the presence of some scavengers of OH radicals

被引:9
作者
Mucka, V. [1 ]
Cervenak, J. [1 ]
Cuba, V. [1 ]
Blaha, P. [1 ]
机构
[1] Czech Tech Univ, Fac Nucl Sci & Phys Engn, CR-11519 Prague 1, Czech Republic
关键词
Ionizing radiation; UV irradiation; Yeast; Bacteria; Radiation protection; HYDROXYL RADICALS; INACTIVATION; CONSEQUENCES; ULTRAVIOLET; PROTECTION; MUTANTS; CELLS;
D O I
10.1007/s10967-014-3696-7
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two new methods for quantitative determination of the role of scavengers of OH radicals in the radiation protection of various microorganisms were studied. The yeast and bacteria were irradiated with gamma or UVC radiations having quite different effect on living cells. Both methods based on the counting of colonies arising from surviving cells proved to be able to describe quantitatively the protective effect of scavengers. The described method may be helpful for study of the radiation protection of cells, radiation stability of DNA or the permeability of the cell membrane.
引用
收藏
页码:237 / 244
页数:8
相关论文
共 23 条
[1]   Identification of new scavengers for hydroxyl radicals and superoxide dismutase by utilising ultraviolet A photoreaction of 8-methoxypsoralen and a variety of mutants of Escherichia coli: Implications on certain diseases of DNA repair deficiency [J].
Ahmad, Shamim I. ;
Yokoi, Masayuki ;
Hanaoka, Fumio .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2012, 116 :30-36
[2]   Long-Term Consequences of Radiation-Induced Bystander Effects Depend on Radiation Quality and Dose and Correlate with Oxidative Stress [J].
Buonanno, Manuela ;
de Toledo, Sonia M. ;
Pain, Debkumar ;
Azzam, Edouard I. .
RADIATION RESEARCH, 2011, 175 (04) :405-415
[3]   Radioprotective efficacy and toxicity of a new family of aminothiol analogs [J].
Copp, Richard R. ;
Peebles, Daniel D. ;
Soref, Cheryl M. ;
Fahl, William E. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2013, 89 (07) :485-492
[4]  
Ewing D., 1987, Radiation Chemistry: Principles and Applications, P501
[5]   Influence of track directions on the biological consequences in cells irradiated with high LET heavy ions [J].
Fujii, Yoshihiro ;
Yurkon, Charles R. ;
Maeda, Junko ;
Genet, Stefan C. ;
Okayasu, Ryuichi ;
Kitamura, Hisashi ;
Fujimori, Akira ;
Kato, Takamitsu A. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2013, 89 (06) :401-410
[6]   QTL for survival to UV-C radiation in Drosophila melanogaster [J].
Gomez, Federico H. ;
Loeschcke, Volker ;
Norry, Fabian M. .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2013, 89 (07) :583-589
[7]   OH Radicals from the Indirect Actions of X-Rays Induce Cell Lethality and Mediate the Majority of the Oxygen Enhancement Effect [J].
Hirayama, Ryoichi ;
Ito, Atsushi ;
Noguchi, Miho ;
Matsumoto, Yoshitaka ;
Uzawa, Akiko ;
Kobashi, Gen ;
Okayasu, Ryuichi ;
Furusawa, Yoshiya .
RADIATION RESEARCH, 2013, 180 (05) :514-523
[8]   Contributions of Direct and Indirect Actions in Cell Killing by High-LET Radiations [J].
Hirayama, Ryoichi ;
Ito, Atsushi ;
Tomita, Masanori ;
Tsukada, Teruyo ;
Yatagai, Fumio ;
Noguchi, Miho ;
Matsumoto, Yoshitaka ;
Kase, Yuki ;
Ando, Koichi ;
Okayasu, Ryuichi ;
Furusawa, Yoshiya .
RADIATION RESEARCH, 2009, 171 (02) :212-218
[9]   Role of Hydroxyl Radicals and Mechanism of Escherichia coli Inactivation on Ag/AgBr/TiO2 Nanotube Array Electrode under Visible Light Irradiation [J].
Hou, Yang ;
Li, Xinyong ;
Zhao, Qidong ;
Chen, Guohua ;
Raston, Colin L. .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, 46 (07) :4042-4050
[10]   Contribution of indirect action to radiation-induced mammalian cell inactivation: Dependence on photon energy and heavy-ion LET [J].
Ito, A ;
Nakano, H ;
Kusano, Y ;
Hirayama, R ;
Furusawa, Y ;
Murayama, C ;
Mori, T ;
Katsumura, Y ;
Shinohara, K .
RADIATION RESEARCH, 2006, 165 (06) :703-712