CYTOKINE SERUM PROFILE IN A GROUP OF SICILIAN NONAGENARIANS

被引：31
作者：
Palmeri, Marisa [1 ]
Misiano, Gabriella [1 ]
Malaguarnera, Mariano [2 ]
Forte, Giusi Irma [1 ]
Vaccarino, Loredana [1 ]
Milano, Salvatore [1 ]
Scola, Letizia [1 ]
Caruso, Calogero [1 ]
Motta, Massimo [2 ]
Maugeri, Domenico [2 ]
Lio, Domenico [1 ]
机构：
[1] Univ Palermo, Dept Biopathol & Med & Forens Biotechnol, I-90134 Palermo, Italy
[2] Univ Catania, Dept Aging Neurol & Urol Sci, Catania, Italy
来源：
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY | 2012年 / 33卷 / 01期
关键词：
circulating cytokine levels; immunoassay; Luminex; serum profile; successful aging; B-CELLS; LONGEVITY; POLYMORPHISM; CENTENARIANS; INFLAMMATION; GENE;
D O I：
10.1080/15321819.2011.601781
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of our study was to evaluate the possibility of using multiplex analysis of the cytokine profile as a marker for successful aging by comparing cytokine plasmatic levels of a group of Sicilian nonagenarians with those of young controls. We analyzed a panel of 17 cytokines, comprehensive of haematopoietic factors T helper 1 (Th1), Th2, inflammation regulatory cytokines, and chemokines. The assay was carried out using the Luminex system. Interleukin (IL)-6 levels (p = 0.01) were increased in nonagenarians, whereas no modifications of other proinflammatory cytokines and chemokines were observed. Interferon-gamma (IFN-gamma) and IL-2 levels are unmodified, suggesting a substantial maintenance of relevant T cell functions. In addition, a significant increase of IL-12 serum levels in nonagenarians versus young controls that might be related to the increase of natural killer (NK) cell functions characterizing aging processes was observed. The analysis of Th2 cytokines show an increase of IL-13 and a reduction of IL-4 levels mirroring the maintenance of some effector's mechanisms of the immunoresponse in advanced ages. Our results suggest that the multiplex analysis of cytokine levels might be useful in defining a successful aging profile.
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页码：82 / 90
页数：9
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