Lack of cross-neutralization by SARS patient sera towards SARS-CoV-2

被引：65

作者：

Anderson, Danielle E. ^{[1
]}

Tan, Chee Wah ^{[1
]}

Chia, Wan Ni ^{[1
]}

Young, Barnaby E. ^{[2
]}

Linster, Martin ^{[1
]}

Low, JennyG H. ^{[3
]}

Tan, Yee-Joo ^{[4
]}

Chen, Mark I-C ^{[2
]}

Smith, Gavin J. D. ^{[1
]}

Leo, Yee Sin ^{[2
]}

Lye, David C. ^{[2
]}

Wang, Lin-Fa ^{[1
]}

机构：

[1] Duke NUS Med Sch, Singapore, Singapore

[2] Natl Ctr Infect Dis, Singapore, Singapore

[3] Singapore Gen Hosp, Singapore, Singapore

[4] Natl Univ Singapore, Singapore, Singapore

来源：

EMERGING MICROBES & INFECTIONS | 2020年 / 9卷 / 01期

基金：

英国医学研究理事会; 新加坡国家研究基金会;

关键词：

SARS; COVID-19; antibody; cross-neutralization; SARS-CoV-2; CORONAVIRUS;

D O I：

10.1080/22221751.2020.1761267

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Despite initial findings indicating that SARS-CoV and SARS-CoV-2 are genetically related belonging to the same virus species and that the two viruses used the same entry receptor, angiotensin-converting enzyme 2 (ACE2), our data demonstrated that there is no detectable cross-neutralization by SARS patient sera against SARS-CoV-2. We also found that there are significant levels of neutralizing antibodies in recovered SARS patients 9-17 years after initial infection. These findings will be of significant use in guiding the development of serologic tests, formulating convalescent plasma therapy strategies, and assessing the longevity of protective immunity for SARS-related coronaviruses in general as well as vaccine efficacy.

引用

页码：900 / 902

页数：3

共 8 条

[1] Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor [J].

Ge, Xing-Yi ;

Li, Jia-Lu ;

Yang, Xing-Lou ;

Chmura, Aleksei A. ;

Zhu, Guangjian ;

Epstein, Jonathan H. ;

Mazet, Jonna K. ;

Hu, Ben ;

Zhang, Wei ;

Peng, Cheng ;

Zhang, Yu-Ji ;

Luo, Chu-Ming ;

Tan, Bing ;

Wang, Ning ;

Zhu, Yan ;

Crameri, Gary ;

Zhang, Shu-Yi ;

Wang, Lin-Fa ;

Daszak, Peter ;

Shi, Zheng-Li .

NATURE, 2013, 503 (7477) :535-+

[2] SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor [J].

Hoffmann, Markus ;

Kleine-Weber, Hannah ;

Schroeder, Simon ;

Krueger, Nadine ;

Herrler, Tanja ;

Erichsen, Sandra ;

Schiergens, Tobias S. ;

Herrler, Georg ;

Wu, Nai-Huei ;

Nitsche, Andreas ;

Mueller, Marcel A. ;

Drosten, Christian ;

Poehlmann, Stefan .

CELL, 2020, 181 (02) :271-+

[3]

Hsu LY, 2003, EMERG INFECT DIS, V9, P713

[4] Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: Sensitive and high-throughput analysis utilizing secreted alkaline phosphatase [J].

Kaku, Yoshihiro ;

Noguchi, Akira ;

Marsh, Glenn A. ;

Barr, Jennifer A. ;

Okutani, Akiko ;

Hotta, Kozue ;

Bazartseren, Boldbaatar ;

Fukushi, Shuetsu ;

Broder, Christopher C. ;

Yamada, Akio ;

Inoue, Satoshi ;

Wang, Lin-Fa .

JOURNAL OF VIROLOGICAL METHODS, 2012, 179 (01) :226-232

[5] Anti-spike IgG causes severe acute lung injury by skewing macrophage responses during acute SARS-CoV infection [J].

Liu, Li ;

Wei, Qiang ;

Lin, Qingqing ;

Fang, Jun ;

Wang, Haibo ;

Kwok, Hauyee ;

Tang, Hangying ;

Nishiura, Kenji ;

Peng, Jie ;

Tan, Zhiwu ;

Wu, Tongjin ;

Cheung, Ka-Wai ;

Chan, Kwok-Hung ;

Alvarez, Xavier ;

Qin, Chuan ;

Lackner, Andrew ;

Perlman, Stanley ;

Yuen, Kwok-Yung ;

Chen, Zhiwei .

JCI INSIGHT, 2019, 4 (04)

[6] Epidemiologic Features and Clinical Course of Patients Infected With SARS-CoV-2 in Singapore [J].

Young, Barnaby Edward ;

Ong, Sean Wei Xiang ;

Kalimuddin, Shirin ;

Low, Jenny G. ;

Tan, Seow Yen ;

Loh, Jiashen ;

Ng, Oon-Tek ;

Marimuthu, Kalisvar ;

Ang, Li Wei ;

Mak, Tze Minn ;

Lau, Sok Kiang ;

Anderson, Danielle E. ;

Chan, Kian Sing ;

Tan, Thean Yen ;

Ng, Tong Yong ;

Cui, Lin ;

Said, Zubaidah ;

Kurupatham, Lalitha ;

Chen, Mark I-Cheng ;

Chan, Monica ;

Vasoo, Shawn ;

Wang, Lin-Fa ;

Tan, Boon Huan ;

Lin, Raymond Tzer Pin ;

Lee, Vernon Jian Ming ;

Leo, Yee-Sin ;

Lye, David Chien .

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2020, 323 (15) :1488-1494

[7] Determination and application of immunodominant regions of SARS coronavirus spike and nucleocapsid proteins recognized by sera from different animal species [J].

Yu, Meng ;

Stevens, Vicky ;

Berry, Jody D. ;

Crameri, Gary ;

McEachern, Jennifer ;

Tu, Changchun ;

Shi, Zhengli ;

Liang, Guodong ;

Weingartl, Hana ;

Cardosa, Jane ;

Eaton, Bryan T. ;

Wang, Lin-Fa .

JOURNAL OF IMMUNOLOGICAL METHODS, 2008, 331 (1-2) :1-12

[8] A pneumonia outbreak associated with a new coronavirus of probable bat origin [J].

Zhou, Peng ;

Yang, Xing-Lou ;

Wang, Xian-Guang ;

Hu, Ben ;

Zhang, Lei ;

Zhang, Wei ;

Si, Hao-Rui ;

Zhu, Yan ;

Li, Bei ;

Huang, Chao-Lin ;

Chen, Hui-Dong ;

Chen, Jing ;

Luo, Yun ;

Guo, Hua ;

Jiang, Ren-Di ;

Liu, Mei-Qin ;

Chen, Ying ;

Shen, Xu-Rui ;

Wang, Xi ;

Zheng, Xiao-Shuang ;

Zhao, Kai ;

Chen, Quan-Jiao ;

Deng, Fei ;

Liu, Lin-Lin ;

Yan, Bing ;

Zhan, Fa-Xian ;

Wang, Yan-Yi ;

Xiao, Geng-Fu ;

Shi, Zheng-Li .

NATURE, 2020, 579 (7798) :270-+

← 1 →