Integrated chemiluminescence-based lab-on-chip for detection of life markers in extraterrestrial environments

被引：34

作者：

Nascetti, Augusto ^{[1
]}

Mirasoli, Mara ^{[2
]}

Marchegiani, Elisa ^{[2
]}

Zangheri, Martina ^{[2
]}

Costantini, Francesca ^{[1
]}

Porchetta, Alessandro ^{[3
]}

Iannascoli, Lorenzo ^{[1
]}

Lovecchio, Nicola ^{[4
]}

Caputo, Domenico ^{[4
]}

de Cesare, Giampiero ^{[4
]}

Pirrotta, Simone ^{[5
]}

Roda, Aldo ^{[2
]}

机构：

[1] Sapienza Univ Rome, Sch Aerosp Engn, Via Salaria 851, I-00138 Rome, Italy

[2] Alma Mater Studiorum Univ Bologna, Dept Chem Giacomo Ciamician, Via Selmi 2, Bologna, Italy

[3] Univ Roma Tor Vergata, Chem Dept, Via Ric Sci, I-00133 Rome, Italy

[4] Sapienza Univ Rome, Dept Informat Engn Elect & Telecommun, Via Eudossiana 18, I-00185 Rome, Italy

[5] Italian Space Agcy, Via Politecn, I-00133 Rome, Italy

来源：

BIOSENSORS & BIOELECTRONICS | 2019年 / 123卷

关键词：

Lab on chip; Life markers; Astrobiology; Thin film photosensors; Chemiluminescence; Adenosine triphosphate; A-CHIP; APTAMER; PERFORMANCE; BIOSENSORS; EVOLUTION; SELECTION; ARRAY; MARS;

D O I：

10.1016/j.bios.2018.08.056

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The detection of life markers is a high priority task in the exploration of the Solar System. Biochips performing in-situ multiplex immunoassays are a very promising approach alternative to gas chromatography coupled with mass spectrometry. As part of the PLEIADES project, we present the development of a chemiluminescence-based, highly integrated analytical platform for the detection of biomarkers outside of the Earth. The PLEIADES device goes beyond the current lab-on-chip approaches that still require bulky external instrumentation for their operation. It exploits an autonomous capillary force-driven microfluidic network, an array of thin-film hydrogenated amorphous silicon photosensors, and chemiluminescence bioassays to provide highly sensitive analyte detection in a very simple and compact configuration. Adenosine triphosphate was selected as the target life marker. Three bioassay formats have been developed, namely (a) a bioluminescence assay exploiting a luciferase mutant with enhanced thermal and pH stability and (b and c) binding assays exploiting antibodies or functional nucleic acids (aptamers) as biospecific recognition elements and peroxidase or DNAzymes as chemiluminescence reporters. Preliminary results, showing limits of detection in the nanomolar range, confirm the validity of the proposed approach.

引用

页码：195 / 203

页数：9