Isolation and characterization of a novel rice gene encoding a putative insect-inducible protein homologous to wheat Wir1

被引:14
作者
Yuan, HY [1 ]
Chen, XP [1 ]
Zhu, L [1 ]
He, GC [1 ]
机构
[1] Wuhan Univ, Coll Life Sci, Key Lab Plant Dev Biol Minist Educ, Wuhan 430072, Peoples R China
基金
中国国家自然科学基金;
关键词
BpHiOO8A gene; brown planthopper; cDNA; Oryza sativa;
D O I
10.1078/0176-1617-01206
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
A full-length cDNA, designated BpHi008A, was cloned representing a rice (Oryza sativa) mRNA that accumulates after brown planthopper (BPH) Nilapar vata lugens Stal feeding. The cDNA encodes a putative 82 amino acid protein (BpHi008A) exhibiting about 37% amino acid sequence identity to Wir1 family of proteins that are encoded by pathogen-induced transcripts in wheat, Like Wir1 proteins, it consists of a hydrophobic N-terminal half and a hydrophilic C-terminal half relatively rich in glycine and proline. These proteins are predicted to be integrated into the membrane, with the C-terminus being extracytoplastic. Genomic Southern analysis indicated that the BpHi008A gene was present as a single-copy sequence in the rice genome, Temporal and spatial studies showed that BpHi008A were systemically induced in rice when 2nd and 3rd-instars were feeding. The BpHi008A transcripts level was also increased in seedlings damaged by mechanical wounding. These data indicated that BphHi008A was implicated in the response of rice plants to BPH feeding and wounding.
引用
收藏
页码:79 / 85
页数:7
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