Anti-inflammatory Effect of Low-Dose Anethole and Ibuprofen Combination Is Accompanied by Partial Prevention of Hepatic Metabolic Changes in Arthritic Rats

被引:4
作者
Ames, Franciele Queiroz [1 ]
Bracht, Livia [2 ]
Lauer Schneider, Larissa Carla [3 ]
Rocha, Bruno Ambrosio [1 ]
Santos, Giovana Alves [1 ]
Lima, Emanuele Parreira [1 ,2 ]
Teobaldo Rocha, Edvalkia Magna [1 ]
Nakamura Cuman, Roberto Kenji [1 ]
Bersani-Amado, Ciomar Aparecida [1 ]
机构
[1] Univ Estadual Maringa, Dept Pharmacol & Therapeut, Ave Colombo 5790, Maringa, Parana, Brazil
[2] Univ Estadual Maringa, Dept Biochem, Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Morphol Sci, Maringa, Parana, Brazil
关键词
anethole; ibuprofen; nonsteroidal anti-inflammatory drug; inflammation; adjuvant-induced arthritis; liver metabolism; INDUCED APOPTOSIS; SKELETAL-MUSCLE; LIVER; GLUCONEOGENESIS; ALPHA;
D O I
10.1007/s10753-020-01241-w
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Anethole (AN) is a natural compound that has attracted great scientific interest because of its numerous biological activities, including anti-inflammatory effects. However, these effects were obtained with high doses of AN, which may be one limitation of its therapeutic use. This study evaluated the effects of a low-dose AN and ibuprofen (IB) combination on inflammatory parameters in Freund's complete adjuvant-induced arthritis (AIA) and arthritis-induced hepatic metabolic changes. Holtzman rats were used and divided into groups: normal, AIA (control), arthritics treated with IB, arthritics treated with AN, and arthritics treated with AN + IB. The volume of the paws, the appearance of secondary lesions, and the number of synovial leukocytes were evaluated. Gluconeogenesis and ureagenesis from alanine were determined in the rat liver in isolated perfusion. The AN + IB (62.5 + 8.75 mg/kg) treatment exerted an inhibitory effect on inflammatory parameters and partially prevented hepatic metabolic changes that was similar to the effect of high-dose IB (35 mg/kg) and AN (250 mg/kg) treatment. This effect of the treatments on hepatic metabolism can be, partly at least, explained by the preservation of both the alanine aminotransferase (ALT) activity and the cytosolic NADH/NAD(+) redox potential in the liver. Taken together, the data obtained provided evidence that the AN + IB combination at lower doses than AN and IB treatment alone had beneficial inhibitory potential for the treatment of AIA and attenuated metabolic changes in the liver. Graphical Abstract
引用
收藏
页码:1680 / 1691
页数:12
相关论文
共 40 条
[1]   Liver glucose metabolism in humans [J].
Adeva-Andany, Maria M. ;
Perez-Felpete, Noemi ;
Fernandez-Fernandez, Carlos ;
Donapetry-Garcia, Cristobal ;
Pazos-Garcia, Cristina .
BIOSCIENCE REPORTS, 2016, 36
[2]  
[Anonymous], METHODS ENZYMATIC AN
[3]  
[Anonymous], 1974, Methods of Enzymatic Analysis. (ed.)
[4]   Combination paracetamol and ibuprofen for pain relief after oral surgery: a dose ranging study [J].
Atkinson, Hartley C. ;
Currie, John ;
Moodie, John ;
Carson, Simon ;
Evans, Steven ;
Worthington, James P. ;
Steenberg, Leon J. ;
Bisley, Eileen ;
Frampton, Chris .
EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 2015, 71 (05) :579-587
[5]   Effect of Aspirin and ibuprofen either alone or in combination on gastric mucosa and bleeding time and on serum prostaglandin E2 and thromboxane A2 levels in the anaesthetized rats in vivo [J].
Bastaki, Salim M. A. ;
Padol, Ireneusz T. ;
Amir, Naheed ;
Hunt, Richard H. .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2018, 438 (1-2) :25-34
[6]  
Bernt Erich, METHODS ENZYMATIC AN, P1205
[7]   Effects of simvastatin, atorvastatin, ezetimibe, and ezetimibe plus simvastatin combination on the inflammatory process and on the liver metabolic changes of arthritic rats [J].
Bracht, Livia ;
Barbosa, Carmem Patricia ;
Caparroz-Assef, Silvana Martins ;
Nakamura Cuman, Roberto Kenji ;
Ishii-Iwamoto, Emy Luiza ;
Bracht, Adelar ;
Bersani-Amado, Ciomar Aparecida .
FUNDAMENTAL & CLINICAL PHARMACOLOGY, 2012, 26 (06) :722-734
[8]   The uncoupling effect of the nonsteroidal anti-inflammatory drug nimesulide in liver mitochondria from adjuvant-induced arthritic rats [J].
Caparroz-Assef, SM ;
Salgueiro-Pagadigorria, CL ;
Bersani-Amado, CA ;
Bracht, A ;
Kelmer-Bracht, AM ;
Ishii-Iwamoto, EL .
CELL BIOCHEMISTRY AND FUNCTION, 2001, 19 (02) :117-124
[9]   Anethole suppressed cell survival and induced apoptosis in human breast cancer cells independent of estrogen receptor status [J].
Chen, Ching Hui ;
deGraffenried, Linda A. .
PHYTOMEDICINE, 2012, 19 (8-9) :763-767
[10]   Protective Mechanism of Anethole on Hepatic Ischemia/Reperfusion Injury in Mice [J].
Cho, Hong-Ik ;
Kim, Kang-Min ;
Kwak, Jong Hwan ;
Lee, Sang Kook ;
Lee, Sun-Mee .
JOURNAL OF NATURAL PRODUCTS, 2013, 76 (09) :1717-1723