N-doped TiO2 based visible light activated label-free photoelectrochemical biosensor for detection of Hg2+ through quenching of photogenerated electrons

被引：19

作者：

Han, Qianqian ^{[1
,2
]}

Wang, Kewei ^{[1
]}

Xu, Lijun ^{[1
]}

Yan, Xiang ^{[1
]}

Zhang, Kunchi ^{[1
]}

Chen, Xing ^{[1
]}

Wang, Qinglin ^{[1
]}

Zhang, Lan ^{[2
]}

Pei, Renjun ^{[1
]}

机构：

[1] Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Collaborat Innovat Ctr Suzhou Nano Sci & Technol, Key Lab Nanobio Interface,Div Nanobiomed, Suzhou 215123, Peoples R China

[2] Xi An Jiao Tong Univ, Sch Mat Sci & Engn, Xian 710049, Peoples R China

来源：

ANALYST | 2015年 / 140卷 / 12期

基金：

中国国家自然科学基金;

关键词：

QUANTUM DOTS; COLORIMETRIC DETECTION; SENSOR; MERCURY(II); GLUTATHIONE; INHIBITION; APTASENSOR; GLUCOSE; IONS;

D O I：

10.1039/c5an00008d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel photoelectrochemical (PEC) biosensor was fabricated based on N-doped TiO2 for the detection of Hg2+ through the quenching of photogenerated electrons. The N-doped TiO2 was synthesized by a sol-gel method with urea and tetrabutyl titanate as the N and Ti sources. Compared with the undoped TiO2, the N-doped TiO2 showed an enhanced photocurrent response under visible light (lambda > 420 nm). The sensing surface was functionalized with 5'-amino-modified T-rich oligonucleotides. The photoelectrochemical biosensor bound Hg2+ on the surface by a highly specific T-Hg2+-T recognition. Hg2+ on the surface of the N-doped TiO2 film withdrew the photogenerated electrons and decreased the recorded current signal. The dynamic linear range for Hg2+ has been determined to be as low as 2-6 mu M.

引用

页码：4143 / 4147

页数：5

共 30 条

[1] Visible-light photocatalysis in nitrogen-doped titanium oxides [J].