Comparative effect of cationic gemini surfactant and its monomeric counterpart on the conformational stability of phospholipase A2

被引：26

作者：

Parray, Mehraj Ud Din ^{[1
]}

Maurya, Neha ^{[1
]}

Wani, Farooq Ahmad ^{[1
]}

Borse, Mahendra S. ^{[2
]}

Arfin, Najmul ^{[1
]}

Malik, Maqsood Ahmad ^{[3
]}

Patel, Rajan ^{[1
]}

机构：

[1] Jamia Millia Islamia, Ctr Interdisciplinary Res Basic Sci, Biophys Chem Lab, New Delhi, India

[2] Uttamrao Patil Coll Dahivel Taluka Sakri, Dept Chem, Dhule, Maharashtra, India

[3] King Abdulaziz Univ, Fac Sci, Dept Chem, POB 80203, Jeddah 21589, Saudi Arabia

来源：

JOURNAL OF MOLECULAR STRUCTURE | 2019年 / 1175卷

关键词：

Gemini surfactants; Phospholipase A(2); Stability; Time resolved fluorescence; Molecular docking; BOVINE SERUM-ALBUMIN; MOLECULAR-DYNAMICS; CIRCULAR-DICHROISM; MEMBRANE-PROTEINS; BINDING; INHIBITION; SIMULATION; INSIGHT; VENOM;

D O I：

10.1016/j.molstruc.2018.07.078

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Herein, we have investigated the effect of cationic gemini surfactant (hexanediyl-alpha,omega-bis-(N-(2-hydroxyethyl) -N-methylhexadecylammonium bromide), (16-6-16MEA) and its monomeric counterpart (N-(2-hyroxyethyl)-N, N-dimethylhexadecylammonium bromide), (16MEA) on the conformational stability of phospholipase A(2) (PLA(2)). The interaction of gemini surfactant and its monomeric counterpart with PLA(2) was characterized by utilizing steady-state fluorescence, time-resolved fluorescence, CD spectroscopy, and computational methods. The steady-state fluorescence results suggested the involvement of static quenching mechanism which was further supported by time resolved fluorescence measurements. The Stern-Volmer equation was utilized to calculate the value of Stern-Volmer quenching constant K-SV. The stoichiometric binding ratio of PLA(2) with 16MEA and 16-6-16MEA was observed to be 1:1. The far-UV CD spectra for PLA(2) revealed similar alteration in secondary structure, in presence of 16MEA and 16-6-16 MEA, which was further supported by computational methods. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：49 / 55

页数：7

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