Psidium guajava']java Flavonoids Prevent NLRP3 Inflammasome Activation and Alleviate the Pancreatic Fibrosis in a Chronic Pancreatitis Mouse Model

被引:6
|
作者
Zhang, Guixian [1 ]
Tang, Liming [2 ]
Liu, Hongbin [1 ]
Liu, Dawei [1 ]
Wang, Manxue [1 ]
Cai, Jun [1 ]
Liu, Weijun [3 ]
Nie, Wei [1 ]
Zhang, Yi [3 ]
Yu, Xiaomeng [1 ]
机构
[1] Tianjin Med & Hlth Res Ctr, Tianjin Inst Med & Pharmaceut Sci, Dept Canc Pharmacol, 79 Duolun Rd, Tianjin 300020, Peoples R China
[2] Tianjin Santan Hosp, Dept Tradit Chinese Med, Tianjin 300020, Peoples R China
[3] Tianjin NanKai Hosp, Tianjin Key Lab Acute Abdomen Dis Associated Orga, Tianjin 300100, Peoples R China
来源
AMERICAN JOURNAL OF CHINESE MEDICINE | 2021年 / 49卷 / 08期
基金
中国国家自然科学基金;
关键词
Total Flavonoids From Psidium Gua[!text type='java']java[!/text] Leaves; Chronic Pancreatitis; Fibrosis; NLRP3; NF-KAPPA-B; EXTRACT; PATHWAY; L; ANTIOXIDANT; MECHANISMS; DIAGNOSIS; BLOCKING;
D O I
10.1142/S0192415X21500944
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Chronic pancreatitis (CP) is a multifactorial, inflammatory syndrome characterized by acinar atrophy and fibrosis. Activation of NOD-like receptors family pyrin domain-containing 3 (NLRP3) inflammasome is a central mediator of multiple chronic inflammatory responses and chronic fibrosis including pancreatic fibrosis in CP. The Psidium guajavaleaf is widely used in traditional medicine for the treatment of chronic inflammation, but the anti-inflammatory effect of Psidium guajavaleaf on CP has not yet been revealed. In this study, we investigated whether the extract of total flavonoids from Psidium guajava leaves (TFPGL) plays a therapeutic mechanism on CP through NLRP3 inflammasome signaling pathway in a mouse CP model. The H&E and acid-Sirius red staining indicted that TFPGL attenuated the inflammatory cell infiltration and fibrosis significantly. The results of immunohistological staining, western blot and RT-qPCR showed that the expressions of NLRP3 and caspase-1 were significantly increased in the CP model group, while TFPGL significantly decreased the NLRP3 and caspase-1 expression at both the gene and protein levels. Moreover, ELISA assay was used to examine the levels of NLRP3 inflammasome target genes, such as caspase-1, IL-1 beta and IL-18. We found that TFPGL treatment decreased the expression of caspase-1, IL-1 beta and IL-18, which is critical for the NLRP3 inflammasome signaling pathway and inflammation response significantly. These results demonstrated that TFPGL attenuated pancreatic inflammation and fibrosis via preventing NLRP3 inflammasome activation and TFPGL can be used as a potential therapeutic agent for CP.
引用
收藏
页码:2001 / 2015
页数:15
相关论文
共 50 条
  • [1] Total Flavonoids From Psidium Guajava']java Leaves Prevent NLRP3 Inflammasome Activation and Alleviate the Pancreatic Fibrosis in a Mouse Model of Chronic Pancreatitis
    Liu, H. B.
    Zhang, G. X.
    PANCREAS, 2017, 46 (10) : 1415 - 1415
  • [2] P2X7R Blockade Prevents NLRP3 Inflammasome Activation and Pancreatic Fibrosis in a Mouse Model of Chronic Pancreatitis
    Zhang, Gui-Xian
    Wang, Man-Xue
    Nie, Wei
    Liu, Da-Wei
    Zhang, Yi
    Liu, Hong-Bin
    PANCREAS, 2017, 46 (10) : 1327 - 1335
  • [3] Flavonoids interfere with NLRP3 inflammasome activation
    Lim, Hyun
    Min, Dong Suk
    Park, Haeil
    Kim, Hyun Pyo
    TOXICOLOGY AND APPLIED PHARMACOLOGY, 2018, 355 : 93 - 102
  • [4] Role of NLRP3 Inflammasome on the Severity of Chronic Pancreatitis in Mouse Models
    Hooda, U.
    Tarique, M.
    Ramakrishnan, V.
    Kashyap, M.
    Cheema, H.
    Bava, E. P.
    Dawra, R.
    Dudeja, V.
    Saluja, A. K.
    PANCREAS, 2018, 47 (10) : 1393 - 1393
  • [5] Systemic Inflammation During Acute Pancreatitis is Regulated by NLRP3 Inflammasome Activation in Pancreatic Macrophages
    Sendler, M.
    Van den Brandt, C.
    Weiss, F. U.
    Golchert, J.
    Homuth, G.
    Lerch, M. M.
    Mayerle, J.
    PANCREAS, 2017, 46 (10) : 1431 - 1431
  • [6] NLRP3 inflammasome activation is required for fibrosis development in NAFLD
    Alexander Wree
    Matthew D. McGeough
    Carla A. Peña
    Martin Schlattjan
    Hongying Li
    Maria Eugenia Inzaugarat
    Karen Messer
    Ali Canbay
    Hal M. Hoffman
    Ariel E. Feldstein
    Journal of Molecular Medicine, 2014, 92 : 1069 - 1082
  • [7] NLRP3 inflammasome activation is required for fibrosis development in NAFLD
    Wree, Alexander
    McGeough, Matthew D.
    Pena, Carla A.
    Schlattjan, Martin
    Li, Hongying
    Inzaugarat, Maria Eugenia
    Messer, Karen
    Canbay, Ali
    Hoffman, Hal M.
    Feldstein, Ariel E.
    JOURNAL OF MOLECULAR MEDICINE-JMM, 2014, 92 (10): : 1069 - 1082
  • [8] Pachymic acid alleviates experimental pancreatic fibrosis through repressing NLRP3 inflammasome activation
    Li, Fu
    Chen, Meng
    Ji, Jianmei
    Tang, Rui
    Huang, Jinxin
    Zhang, Xiwen
    Yu, Jinzhong
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2022, 86 (11) : 1497 - 1505
  • [9] Flavonoids with Inhibitory Effects on NLRP3 Inflammasome Activation from Millettia velutina
    Ma, Xu
    Zhao, Min
    Tang, Ming-Hai
    Xue, Lin-Lin
    Zhang, Rui-Jia
    Liu, Ling
    Ni, Heng-Fan
    Cai, Xiao-Ying
    Kuang, Shuang
    Hong, Feng
    Wang, Lun
    Chen, Kai
    Tang, Huan
    Li, Yan
    Peng, Ai-Hua
    Yang, Jian-Hong
    Pei, He-Ying
    Ye, Hao-Yu
    Chen, Li-Juan
    JOURNAL OF NATURAL PRODUCTS, 2020, 83 (10): : 2950 - 2959
  • [10] NLRP3 inflammasome activation in the thymus of MPTP-induced Parkinsonian mouse model
    Wen, Lu
    Zhang, Qiu-Shuang
    Heng, Yang
    Chen, Ying
    Wang, Shuo
    Yuan, Yu-He
    Chen, Nai-Hong
    TOXICOLOGY LETTERS, 2018, 288 : 1 - 8