An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection

被引：127

作者：

Wang, Libing ^{[1
,2
]}

Ma, Wenwei ^{[1
]}

Chen, Wei ^{[1
]}

Liu, Liqiang ^{[1
]}

Ma, Wei ^{[1
]}

Zhu, Yingyue ^{[1
]}

Xu, Liguang ^{[1
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[2] Hunan Entry Exit Inspect & Quarantine Bur, Changsha 410001, Hunan, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2011年 / 26卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Aptamer; Gold nanoparticle; Strip; Ochratoxin A; LINKED-IMMUNOSORBENT-ASSAY; OCHRATOXIN-A; IMMUNOSENSOR; MICROCYSTIN; MOLECULES;

D O I：

10.1016/j.bios.2010.11.040

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：3059 / 3062

页数：4

共 25 条

[1] Evaluation of methods used to determine ochratoxin a in coffee beans [J].