Insulin-like growth factor-binding protein 6 inhibits survival and differentiation of rat oligodendrocyte precursor cells

被引:20
作者
Kühl, NM
Hoekstra, D
De Vries, H
De Keyser, J
机构
[1] Acad Hosp Groningen, Dept Neurol, NL-9700 RB Groningen, Netherlands
[2] Univ Groningen, Dept Membrane Cell Biol, Groningen, Netherlands
关键词
O2A cells; survival; myelin proteins; mRNA; des(1-3)-IGF-1;
D O I
10.1002/glia.10263
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Insulin-like growth factor 1 (IGF-1) is a growth and survival factor for oligodendrocyte lineage cells and promotes myelination. We demonstrate that IGF-binding protein 6 (IGFBP-6) is expressed and localized to the Golgi complex in rat oligodendrocyte precursor (O2A) cells. IGFBP-6 mRNA showed a developmentally regulated expression pattern, displaying a transient decrease during early development, and enhanced levels upon cell maturation. IGFBP-6 mRNA expression could be reduced by addition of basic fibroblast growth factor and progesterone while estrogen increased IGFBP-6 mRNA. IGF-1, platelet-derived growth factor, and insulin had no effect. When added exogenously, IGFBP-6 reduced O2A cell survival in the absence of IGF-1 and inhibited IGF-1-stimulated survival in a partially IGF-1-dependent and partially IGF-1-independent fashion. In addition, IGFBP-6 reduced the IGF-stimulated expression of two myelin proteins, CNPase and MAG. Taken together, the data show that IGFBP-6 is a new negative effector of oligodendrocyte survival and differentiation. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:91 / 101
页数:11
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