Identification of "Cronobacter" spp. (Enterobacter sakazakii)

被引：84
作者：
Iversen, Carol
Lehner, Angelika
Mullane, Niall
Marugg, John
Fanning, Seamus
Stephan, Roger
Joosten, Han
机构：
[1] Univ Zurich, Vetsuisse Fac, Inst Food Safety & Hyg, CH-8057 Zurich, Switzerland
[2] Nestle Res Ctr, Qual & Safety Dept, CH-1000 Lausanne, Switzerland
[3] Univ Coll Dublin, UCD Vet Sci Ctr, Ctr Food Safety, Dublin, Ireland
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 11期
关键词：
D O I：
10.1128/JCM.01026-07
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A taxonomic reclassification of the neonatal pathogen Enterobacter sakazakii to consist of five species within a new genus, "Cronobacter," has recently been proposed. The correct identification of these organisms is important to clinicians. Therefore, using 312 Enterobacteriaceae, including 210 "Cronobacter" strains, the reliabilities of biochemical and genetic confirmation tests were investigated. All "Cronobacter" isolates were positive using dnaG and gluA PCR protocols, and all expressed alpha-glucosidase activity. ID32E v3.0 identified 99.5% of "Cronobacter" isolates (as the nearest match to E. sakazakii).
引用
收藏
页码：3814 / 3816
页数：3
相关论文
共 21 条
[1]  
Besse NG, 2006, J AOAC INT, V89, P1309
[2]   Characterization of a collection of Enterobacter sakazakii isolates from environmental and food sources [J].
Drudy, Denise ;
O'Rourke, Michele ;
Murphy, Mary ;
Mullane, Niall R. ;
O'Mahony, Rebecca ;
Kelly, Lorraine ;
Fischer, Matthias ;
Sanjaq, Suhad ;
Shannon, Pauline ;
Wall, Patrick ;
O'Mahony, Micheal ;
Whyte, Paul ;
Fanning, Seamus .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2006, 110 (02) :127-134
[3]   Comparison of the phenotyping methods ID 32E and VITEK 2 compact GN with 16S rRNA gene sequencing for the identification of Enterobacter sakazakii [J].
Fanjat, Nadege ;
Leclercq, Alexandre ;
Joosten, Han ;
Robichon, Denis .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (06) :2048-2050
[4]   ENTEROBACTER-SAKAZAKII - A NEW SPECIES OF ENTEROBACTERIACEAE ISOLATED FROM CLINICAL SPECIMENS [J].
FARMER, JJ ;
ASBURY, MA ;
HICKMAN, FW ;
BRENNER, DJ .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1980, 30 (03) :569-584
[5]  
*INT ORG STAND, 2006, 22964 ISO TS
[6]   Identification and phylogeny of Enterobacter sakazakii relative to Enterobacter and Citrobacter species [J].
Iversen, C ;
Waddington, M ;
On, SLW ;
Forsythe, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (11) :5368-5370
[7]   A selective differential medium for Enterobacter sakazakii, a preliminary study [J].
Iversen, C ;
Druggan, P ;
Forsythe, S .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2004, 96 (02) :133-139
[8]   The taxonomy of Enterobacter sakazakii:: proposal of a new genus Cronobacter gen. nov and descriptions of Cronobacter sakazakii comb. nov Cronobacter sakazakii subsp sakazakii, comb. nov., Cronobacter sakazakii subsp malonaticus subsp nov., Cronobacter turicensis sp nov., Cronobacter muytjensii sp nov., Cronobacter dublinensis sp nov and Cronobacter genomospecies I [J].
Iversen, Carol ;
Lehner, Angelika ;
Mullane, Niall ;
Bidlas, Eva ;
Cleenwerck, Ilse ;
Marugg, John ;
Fanning, Seamus ;
Stephan, Roger ;
Joosten, Han .
BMC EVOLUTIONARY BIOLOGY, 2007, 7 (1)
[9]  
KAMPFER P, 1991, J CLIN MICROBIOL, V29, P2877
[10]   Comparison of two chromogenic media and evaluation of two molecular based identification systems for Enterobacter sakazakii detection [J].
Lehner, A ;
Nitzsche, S ;
Breeuwer, P ;
Diep, B ;
Thelen, K ;
Stephan, R .
BMC MICROBIOLOGY, 2006, 6 (1) :8P
123