New technology to examine and improve the purity of hybrid rice with herbicide resistant gene

被引：3

作者：

Huang, DN ^{[1
]}

Li, JY ^{[1
]}

Zhang, SQ ^{[1
]}

Xue, R ^{[1
]}

Yang, W ^{[1
]}

Hua, ZH ^{[1
]}

Xie, XB ^{[1
]}

Wang, XL ^{[1
]}

机构：

[1] China Natl Rice Res Inst, Hangzhou 310006, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 1998年 / 43卷 / 09期

关键词：

herbicide resistant bar gene; transgenic hybrid rice; purity of hybrid rice;

D O I：

10.1007/BF02898961

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The herbicide resistant bar gene has been widely used as selectable marker genes in the study on plant genetic transformation. Owing to the integration of the gene into rice genome, transgenic rice was resistant to the herbicide Basta. Therefore, selection of transgenic plant and genetic analysis became easier. In the studies, bar gene was introduced as a genetic marker gene into rice restore line variety of two-line or three-line. Combined with conventional breeding method, the good herbicide resistant individual plant was derived as the new restore line for hybrid combination. After sprayed with Basta at seedling phase, the real hybrid rice that had herbicide resistance could grow normally, while the fake hybrid rice and weeds, having no bar gene, were killed by herbicide. The method described above helped rapidly to examine and improve the purity of hybrid rice.

引用

页码：784 / 787

页数：4