Infant formula;
Heat treatment;
Milk protein structure;
Dynamic in vitro digestion;
GASTRIC DIGESTION;
PEPTIDE RELEASE;
PASTEURIZATION;
GOAT;
COW;
D O I:
10.1016/j.foodhyd.2021.107368
中图分类号:
O69 [应用化学];
学科分类号:
081704 ;
摘要:
The impact of protein structure in model infant milk formulas (IMFs) on digestion was evaluated using an in vitro dynamic digestion model. IMFs differed by their whey protein profile and the heating conditions. Digesta microstructure was followed by laser light scattering and confocal laser scanning microscopy. Proteolysis kinetics were monitored by SDS-PAGE and OPA assay and peptide release kinetics by tandem mass spectrometry. The gastric digesta microstructure depended on the protein structure within IMFs, with the highest protein particle size in IMF containing native casein micelles. Among whey proteins, denatured/aggregated lactoferrin was the most sensitive to pepsin hydrolysis. The peptidomic profile during digestion varied among IMFs. More numerous and resistant intestinal bioactive peptides were observed for the unheated IMF having a whey protein profile closer to that of human milk. Further physiological studies are required to investigate the benefits of adapting the IMF whey protein profile on infant health.
机构:
Fonterra Res & Dev Ctr, Private Bag 11029,Dairy Farm Rd, Palmerston North, New Zealand
Massey Univ, Riddet Inst, Private Bag 11222, Palmerston North 4442, New ZealandFonterra Res & Dev Ctr, Private Bag 11029,Dairy Farm Rd, Palmerston North, New Zealand
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
de Oliveira, Samira. C.
;
Deglaire, Amelie
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Deglaire, Amelie
;
Menard, Olivia
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Menard, Olivia
;
Bellanger, Amandine
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Univ Rennes, Dept Pediat, 16 Blvd Bulgarie, F-35203 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Bellanger, Amandine
;
Rousseau, Florence
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Rousseau, Florence
;
Henry, Gwenaele
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Henry, Gwenaele
;
Dirson, Emelyne
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Univ Rennes, Dept Pediat, 16 Blvd Bulgarie, F-35203 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Dirson, Emelyne
;
Carriere, Frederic
论文数: 0引用数: 0
h-index: 0
机构:
Aix Marseille Univ, CNRS, UMR Enzymol Interfaciale & Physiol Lipolyse 7282, 31 Chemin Joseph Aiguier, F-13402 Marseille, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Carriere, Frederic
;
Dupont, Didier
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Dupont, Didier
;
Bourlieu, Claire
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
机构:
Fonterra Res & Dev Ctr, Private Bag 11029,Dairy Farm Rd, Palmerston North, New Zealand
Massey Univ, Riddet Inst, Private Bag 11222, Palmerston North 4442, New ZealandFonterra Res & Dev Ctr, Private Bag 11029,Dairy Farm Rd, Palmerston North, New Zealand
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
de Oliveira, Samira. C.
;
Deglaire, Amelie
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Deglaire, Amelie
;
Menard, Olivia
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Menard, Olivia
;
Bellanger, Amandine
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Univ Rennes, Dept Pediat, 16 Blvd Bulgarie, F-35203 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Bellanger, Amandine
;
Rousseau, Florence
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Rousseau, Florence
;
Henry, Gwenaele
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Henry, Gwenaele
;
Dirson, Emelyne
论文数: 0引用数: 0
h-index: 0
机构:
Ctr Hosp Univ Rennes, Dept Pediat, 16 Blvd Bulgarie, F-35203 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Dirson, Emelyne
;
Carriere, Frederic
论文数: 0引用数: 0
h-index: 0
机构:
Aix Marseille Univ, CNRS, UMR Enzymol Interfaciale & Physiol Lipolyse 7282, 31 Chemin Joseph Aiguier, F-13402 Marseille, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Carriere, Frederic
;
Dupont, Didier
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Dupont, Didier
;
Bourlieu, Claire
论文数: 0引用数: 0
h-index: 0
机构:
INRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France
Agrocampus Ouest, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, FranceINRA, UMR 1253, Sci & Technol Lait & Oeuf, 65 Rue St Brieuc, F-35042 Rennes, France