Role of Nesfatin-1 in the Reproductive Axis of Male Rat

被引：45

作者：

Gao, Xiaoxiao ^{[1
]}

Zhang, Kaifa ^{[1
]}

Song, Min ^{[1
,2
,3
]}

Li, Xiumei ^{[1
,2
,3
]}

Luo, Lei ^{[1
]}

Tian, Yuan ^{[1
]}

Zhang, Yunhai ^{[1
,2
,3
]}

Li, Yunsheng ^{[1
,2
,3
]}

Zhang, Xiaorong ^{[1
,2
,3
]}

Ling, Yinghui ^{[1
,2
,3
]}

Fang, Fugui ^{[1
,2
,3
]}

Liu, Ya ^{[1
,2
,3
]}

机构：

[1] Anhui Agr Univ, Coll Anim Sci & Technol, Anhui Prov Lab Anim Genet Resources Protect & Bre, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

[2] Anhui Prov Lab Local Livestock & Poultry Genet Re, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

[3] Anhui Agr Univ, Coll Anim Sci & Technol, Dept Anim Vet Sci, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

中国国家自然科学基金;

关键词：

DEHYDROEPIANDROSTERONE-SULFATE; FUNCTIONAL-ROLE; EXPRESSION; TESTOSTERONE; KISSPEPTIN; NUCB2/NESFATIN-1; PUBERTY; STEROIDOGENESIS; METABOLISM; SECRETION;

D O I：

10.1038/srep32877

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nesfatin-1 is an important molecule in the regulation of reproduction. However, its role in the reproductive axis in male animals remains to be understood. Here, we found that nesfatin-1 was mainly distributed in the arcuate nucleus (ARC), paraventricular nucleus (PVN), periventricular nucleus (PeN), and lateral hypothalamic area (LHA) of the hypothalamus; adenohypophysis and Leydig cells in male rats. Moreover, the concentrations of serum nesfatin-1 and its mRNA in hypothalamo-pituitary-gonadal axis (HPGA) vary with the age of the male rat. After intracerebroventricular injection of nesfatin-1, the hypothalamic genes for gonadotrophin releasing hormone (GnRH), kisspeptin (Kiss-1), pituitary genes for follicle-stimulate hormone beta(FSH beta), luteinizing hormone beta(LH beta), and genes for testicular steroidogenic acute regulatory (StAR) expression levels were decreased significantly. Nesfatin-1 significantly increased the expression of genes for 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD), and cytochrome P450 cleavage (P450scc) in the testis of pubertal rats, but their levels decreased in adult rats (P < 0.05), along with the serum FSH, LH, and testosterone (T) concentrations. After nesfatin-1 addition in vitro, T concentrations of the supernatant were significantly higher than that in the control group. These results were suggestive of the role of nesfatin-1 in the regulation of the reproductive axis in male rats.

引用

页数：10

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