Bimetallic Pd@Au nanorods based ultrasensitive acetylcholinesterase biosensor for determination of organophosphate pesticides

被引：98
作者：
Lu, Xiong [1 ]
Tao, Lu [1 ]
Song, Dandan [1 ]
Li, Yan [1 ]
Gao, Faming [1 ]
机构：
[1] Yanshan Univ, Dept Appl Chem, Key Lab Appl Chem, Qinhuangdao 066004, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2018年 / 255卷
基金：
中国国家自然科学基金;
关键词：
Biosensor; Nanorod; Core-shell; Pd@Au; GOLD NANORODS; GRAPHENE OXIDE; CHROMATOGRAPHY/MASS SPECTROMETRY; MODIFIED ELECTRODE; CARBON NANOTUBES; PRUSSIAN BLUE; NANOPARTICLES; NANOCOMPOSITE; FILM; IMMOBILIZATION;
D O I：
10.1016/j.snb.2017.09.063
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
One-dimensional bimetallic Pd@Au core-shell nanorods (NRs) with uniform Au shell were synthesized and applied to construct a highly sensitive acetylcholinesterase (AChE) biosensor for the detection of organophosphate pesticides (OPs). The Pd@Au core-shell nanorods with excellent conductivity, pure contact surface and good biocompatibility can provide an extremely suitable area for the adhesion of AChE. Due to these characteristics, the constructed sensor exhibits a superior performance. The biosensor was prepared via a simple procedure and its optimization studies were discussed. Under optimal conditions, the inhibition of paraoxon (as a model compound for OPs) in linearly proportional to its concentration in the range of 3.6 pM-100 nM, with the detection limit of 3.6 pM (calculated for 10% inhibition). The KMapp value of the fabricated enzyme electrode was calculated to be 0.207 mM. The application of this biosensor in the analysis of real samples was also evaluated. Moreover, the prepared biosensor possessed higher sensitivity and acceptable reproducibility and stability, showing great potential in the detection of OPs. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：2575 / 2581
页数：7
相关论文
共 44 条
[1]   Recent advances in biosensors based on enzyme inhibition [J].
Amine, A. ;
Arduini, F. ;
Moscone, D. ;
Palleschi, G. .
BIOSENSORS & BIOELECTRONICS, 2016, 76 :180-194
[2]   Acetylcholinesterase biosensor based on self-assembled monolayer-modified gold-screen printed electrodes for organophosphorus insecticide detection [J].
Arduini, Fabiana ;
Guidone, Simone ;
Amine, Aziz ;
Palleschi, Giuseppe ;
Moscone, Danila .
SENSORS AND ACTUATORS B-CHEMICAL, 2013, 179 :201-208
[3]   High-Performance Liquid Chromatographic Determination of Diazinon after Its Magnetic Dispersive Solid-Phase Microextraction Using Magnetic Molecularly Imprinted Polymer [J].
Bazmandegan-Shamili, Alireza ;
Dadfarnia, Shayessteh ;
Shabani, Ali Mohammad Haji ;
Saeidi, Mahboubeh ;
Moghadam, Masoud Rohani .
FOOD ANALYTICAL METHODS, 2016, 9 (09) :2621-2630
[4]   In Silico Pharmacophore Model for Tabun-Inhibited Acetylcholinesterase Reactivators: A Study of Their Stereoelectronic Properties [J].
Bhattacharjee, Apurba K. ;
Kuca, Kamil ;
Musilek, Kamil ;
Gordon, Richard K. .
CHEMICAL RESEARCH IN TOXICOLOGY, 2010, 23 (01) :26-36
[5]   A Novel Acetylcholinesterase Biosensor: Core-Shell Magnetic Nanoparticles Incorporating a Conjugated Polymer for the Detection of Organophosphorus Pesticides [J].
Cancar, Hurija Dzudzevic ;
Soylemez, Saniye ;
Akpinar, Yeliz ;
Kesik, Melis ;
Goker, Seza ;
Gunbas, Gorkem ;
Volkan, Murvet ;
Toppare, Levent .
ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (12) :8058-8067
[6]   Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity [J].
Connor, EE ;
Mwamuka, J ;
Gole, A ;
Murphy, CJ ;
Wyatt, MD .
SMALL, 2005, 1 (03) :325-327
[7]   An acetylcholinesterase biosensor for determination of low concentrations of Paraoxon and Dichlorvos [J].
Di Tuoro, D. ;
Portaccio, M. ;
Lepore, M. ;
Arduini, F. ;
Moscone, D. ;
Bencivenga, U. ;
Mita, D. G. .
NEW BIOTECHNOLOGY, 2011, 29 (01) :132-138
[8]   Immobilization of acetylcholinesterase on gold nanoparticles embedded in sol-gel film for amperometric detection of organophosphorous insecticide [J].
Du, Dan ;
Chen, Shizhen ;
Cai, Jie ;
Zhang, Aidong .
BIOSENSORS & BIOELECTRONICS, 2007, 23 (01) :130-134
[9]   Amperometric biosensing of organophosphate and organocarbamate pesticides utilizing polypyrrole entrapped acetylcholinesterase electrode [J].
Dutta, Rekha Rani ;
Puzari, Panchanan .
BIOSENSORS & BIOELECTRONICS, 2014, 52 :166-172
[10]   Disposable pipette extraction for the analysis of pesticides in fruit and vegetables using gas chromatography/mass spectrometry [J].
Guan, Hongxia ;
Brewer, William E. ;
Garris, Sherry T. ;
Morgan, Stephen L. .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (12) :1867-1874
12345