Reprogramming rat embryonic fibroblasts into induced pluripotent stem cells using transposon vectors and their chondrogenic differentiation in vitro

被引：8
作者：
Ye, Jiakuan [1 ]
Hong, Junyi [1 ]
Ye, Fusheng [1 ]
机构：
[1] Xiaoshan Hosp, Dept Orthopaed, Hangzhou 311202, Zhejiang, Peoples R China
来源：
MOLECULAR MEDICINE REPORTS | 2015年 / 11卷 / 02期
关键词：
reprogramming; piggyBac transposon; induced pluripotent stem cells; mPB; chondrocyte; MOUSE FIBROBLASTS; DEFINED FACTORS; GENERATION; PROGENITORS; CONVERSION;
D O I：
10.3892/mmr.2014.2793
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
It has been known that the successful reprogramming of differentiated human somatic cells into a pluripotent state would allow for the creation of cartilage cells. However, current virus-mediated strategies to form induced pluripotent stem cells (iPSCs) are limited in their clinical application due to exogenous gene modification. In the present study, the piggyBac transposon system carrying corresponding genes (Oct3/4, Klf4, Sox2 and c-Myc) was employed to reprogram rat embryonic fibroblasts (REFs) into iPSCs, and the transposon-carried genes were successfully removed by a transposase system. Furthermore, the differentiation capacity of the iPSCs into chondrocytes was investigated in vitro. A typical chondrocyte marker, collagen-II, was expressed following culture. In conclusion, rat iPSCs without genetic modification were obtained from REFs using the piggyBac transposon system and the transposase mPB system, and these cells possessed the capacity of chondrocyte differentiation, suggesting that this method may be an effective approach for the treatment of cartilage disorders in the future.
引用
收藏
页码：989 / 994
页数：6
相关论文
共 29 条
[1]   Conversion of mouse fibroblasts into cardiomyocytes using a direct reprogramming strategy [J].
Efe, Jem A. ;
Hilcove, Simon ;
Kim, Janghwan ;
Zhou, Hongyan ;
Ouyang, Kunfu ;
Wang, Gang ;
Chen, Ju ;
Ding, Sheng .
NATURE CELL BIOLOGY, 2011, 13 (03) :215-U61
[2]  
Fukunaga N, 2010, CELL REPROGRAM, V12, P369, DOI [10.1089/cell.2009.0097, 10.1089/cell.2009.0086]
[3]   Efficient differentiation of human iPSC-derived mesenchymal stem cells to chondroprogenitor cells [J].
Guzzo, Rosa M. ;
Gibson, Jason ;
Xu, Ren-He ;
Lee, Francis Y. ;
Drissi, Hicham .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2013, 114 (02) :480-490
[4]   Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin [J].
Hanna, Jacob ;
Wernig, Marius ;
Markoulaki, Styliani ;
Sun, Chiao-Wang ;
Meissner, Alexander ;
Cassady, John P. ;
Beard, Caroline ;
Brambrink, Tobias ;
Wu, Li-Chen ;
Townes, Tim M. ;
Jaenisch, Rudolf .
SCIENCE, 2007, 318 (5858) :1920-1923
[5]   Hematopoietic stem cells convert into liver cells within days without fusion [J].
Jang, YY ;
Collector, MI ;
Baylin, SB ;
Diehl, AM ;
Sharkis, SJ .
NATURE CELL BIOLOGY, 2004, 6 (06) :532-539
[6]   Extrinsic factors derived from mouse embryonal carcinoma cell lines maintain pluripotency of mouse embryonic stem cells through a novel signal pathway [J].
Kawazoe, Shinjirou ;
Ikeda, Nobuhito ;
Miki, Kengo ;
Shibuya, Masayuki ;
Morikawa, Kumi ;
Nakano, Seiji ;
Oshimura, Mitsuo ;
Hisatome, Ichiro ;
Shirayoshi, Yasuaki .
DEVELOPMENT GROWTH & DIFFERENTIATION, 2009, 51 (02) :81-93
[7]   Direct reprogramming of mouse fibroblasts to neural progenitors [J].
Kim, Janghwan ;
Efe, Jem A. ;
Zhu, Saiyong ;
Talantova, Maria ;
Yuan, Xu ;
Wang, Shufen ;
Lipton, Stuart A. ;
Zhang, Kang ;
Ding, Sheng .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 (19) :7838-7843
[8]   Human Induced Pluripotent Stem Cells Differentiated into Chondrogenic Lineage Via Generation of Mesenchymal Progenitor Cells [J].
Koyama, Noriaki ;
Miura, Masako ;
Nakao, Kazumasa ;
Kondo, Eri ;
Fujii, Toshihito ;
Taura, Daisuke ;
Kanamoto, Naotetsu ;
Sone, Masakatsu ;
Yasoda, Akihiro ;
Arai, Hiroshi ;
Bessho, Kazuhisa ;
Nakao, Kazuwa .
STEM CELLS AND DEVELOPMENT, 2013, 22 (01) :102-113
[9]   Embryonic stem cell-derived chondrogenic differentiation in vitro:: activation by BMP-2 and BMP-4 [J].
Kramer, J ;
Hegert, C ;
Guan, KM ;
Wobus, AM ;
Müller, PK ;
Rohwedel, J .
MECHANISMS OF DEVELOPMENT, 2000, 92 (02) :193-205
[10]   Chondrogenic Differentiation in vitro of Murine Two-Factor Induced Pluripotent Stem Cells is Comparable to Murine Embryonic Stem Cells [J].
Kuboth, Sina ;
Kramer, Jan ;
Rohwedel, Juergen .
CELLS TISSUES ORGANS, 2012, 196 (06) :481-489
123