Useful In Vitro Techniques to Evaluate the Mucoadhesive Properties of Hyaluronic Acid-Based Ocular Delivery Systems

被引:62
作者
Graca, Angelica [1 ]
Goncalves, Lidia Maria [2 ]
Raposo, Sara [1 ,2 ]
Ribeiro, Helena Margarida [2 ]
Marto, Joana [1 ,2 ]
机构
[1] Lab Edol Prod Farmaceut SA, P-2595225 Linda A Velha, Portugal
[2] Univ Lisbon, Fac Pharm, Res Inst Med iMed ULisboa, P-1649003 Lisbon, Portugal
关键词
mucoadhesion; in vitro tests; hyaluronic acid; eye drops; dry eye disease; medical devices; FILM LIPID LAYER; DRY EYE DISEASE; TEAR FILM; TENSILE-STRENGTH; PH;
D O I
10.3390/pharmaceutics10030110
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Polymer-based eye drops are the most used drug delivery system to treat dry eye disease (DED). Therefore, the mucoadhesion between the polymer and the ocular mucin is crucial to ensure the efficacy of the treatment. In this context, the present study aimed to evaluate the potential use of in vitro methods to study the mucoadhesion of eye drop solutions and, specifically to evaluate the efficacy of two hyaluronic acid-based formulations (HA), HA 0.15% and 0.30% (w/v) to treat DED. Rheology methods and zeta potential determination were used to study the mucoadhesive properties of both eye drop solutions. All results indicated that interactions occurred between the mucin and the HA, being stronger with HA 0.30%, due to the physical entanglements and hydrogen bounding. In vitro tests on ARPE-19 cell line were performed using a 2D and a 3D dry eye model and the results have shown that pre-treated cells with HA showed a morphology more similar to the hydrated cells in both products, with a high survival rate. The in vitro techniques used in this study have been shown to be suitable to evaluate and predict mucoadhesive properties and the efficacy of the eye drops on relief or treatment of DED. The results obtained from these methods may help in inferring possible in vivo effects.
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页数:16
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