Isolation and genetic characterization of Nocardia seriolae from snubnose pompano Trachinotus blochii in Vietnam

被引：41

作者：

Hung Vu-Khac ^{[1
]}

Van Quy Binh Duong ^{[1
]}

Chen, Shih-Chu ^{[2
]}

Trung Hieu Pham ^{[1
]}

Thi Thu Giang Nguyen ^{[1
]}

Thi Thu Hang Trinh ^{[1
]}

机构：

[1] Cent Vietnam Vet Inst, Dept Biotechnol, Km4 Dong De St, Nha Trang, Vietnam

[2] Natl Pingtung Univ Sci & Technol, Coll Vet Med, Dept Vet Med, Pingtung 912, Taiwan

来源：

DISEASES OF AQUATIC ORGANISMS | 2016年 / 120卷 / 02期

关键词：

Pathogenicity; Nocardiosis; Snubnose pompano; Aquaculture; FISH PATHOGEN; IDENTIFICATION; INFECTION;

D O I：

10.3354/dao03023

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

A total of 480 cage-cultured fish were collected from 4 coastal provinces in central Vietnam to investigate the causative agent of nocardiosis. Fish displayed unique characteristics such as paleness and lethargy and exhibited haemorrhages and ulcers on the skin. Prominent white nodules varying in size were observed in the spleen, kidney, and liver. Furthermore, histopathological sections showed typical granulomatous lesions in these organs. Using the Ziehl-Neelsen staining method, isolated bacteria exhibited acid-fast, bead-like filament morphology when cultured in brain-heart infusion medium or Ogawa medium. Phylogenetic analysis of 16S rDNA confirmed that the isolated bacterium was Nocardia seriolae. This study demonstrates for the first time an outbreak of N. seriolae in snubnose pompano in central Vietnam.

引用

页码：173 / 177

页数：5

共 23 条

[1] Nocardiosis in sea bass, Lateolabrax japonicus, in Taiwan [J].

Chen, SC ;

Lee, JL ;

Lai, CC ;

Gu, YW ;

Wang, CT ;

Chang, HY ;

Tsai, KH .

JOURNAL OF FISH DISEASES, 2000, 23 (05) :299-307

[2]

FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x

[3]

Giang N.T.T., 2012, J. Fish. Sci. Technol., V4, P26

[4]

Jukes TH., 1969, MAMMALIAN PROTEIN ME, P21, DOI [DOI 10.1016/B978-1-4832-3211-9.50009-7, DOI 10.1093/BIOINFORMATICS/BTM404, 10.1016/B978-1-4832-3211-9.50009-7]

[5]

Kariya T., 1968, Fish Pathol, V3, P16, DOI [10.3147/jsfp.3.16, DOI 10.3147/JSFP.3.16]

[6] Sequencing of 16S-23S rRNA internal transcribed spacer and its application in the identification of Nocardia seriolae by polymerase chain reaction [J].

Kono, T ;

Ooyama, T ;

Chen, SC ;

Sakai, M .

AQUACULTURE RESEARCH, 2002, 33 (14) :1195-1197

[7]

Kubota S, 1968, FISH PATHOL, V3, P24, DOI DOI 10.3147/JSFP.3.24

[8] NOCARDIA-SERIOLAE SP-NOV CAUSING NOCARDIOSIS OF CULTURED FISH [J].

KUDO, T ;

HATAI, K ;

SEINO, A .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1988, 38 (02) :173-178

[9]

Kumamoto H, 1985, B NIPPON VET ZOOTECH, V34, P100

[10]

KUSUDA R, 1974, B JPN SOC SCI FISH, V40, P369

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