Insulin-like growth factor-1 endues monocytes with immune suppressive ability to inhibit inflammation in the intestine

被引：43

作者：

Ge, Rong-Ti ^{[1
]}

Mo, Li-Hua ^{[2
,3
]}

Wu, Ruijin ^{[1
]}

Liu, Jiang-Qi ^{[4
]}

Zhang, Huan-Ping ^{[4
]}

Liu, Zhigang ^{[1
,2
,3
]}

Liu, Zhanju

Yang, Ping-Chang ^{[2
,3
,4
]}

机构：

[1] Tongji Univ, Shanghai Peoples Hosp 10, Dept Gastroenterol, Shanghai 200072, Peoples R China

[2] Shenzhen Univ, Sch Med, Shenzhen Key Lab Allergy & Immunol, Shenzhen 518060, Peoples R China

[3] Shenzhen Univ, State Key Lab Resp Dis Allergy, Shenzhen 518060, Peoples R China

[4] McMaster Univ, Brain Body Inst, Hamilton, ON L8N 4A6, Canada

来源：

SCIENTIFIC REPORTS | 2015年 / 5卷

关键词：

T-CELLS; EXPERIMENTAL COLITIS; BOWEL-DISEASE; IMMUNOGLOBULIN; HOMEOSTASIS; SECRETION; RECEPTOR; ALLERGY; MODEL;

D O I：

10.1038/srep07735

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The pathogenesis of some chronic inflammation such as inflammatory bowel disease is unclear. Insulin-like growth factor-1 (IGF1) has active immune regulatory capability. This study aims to investigate into the mechanism by which IGF1 modulates the monocyte (Mo) properties to inhibit immune inflammation in the intestine. In this study, the production of IGF1 by intestinal epithelial cells was evaluated by real time RT-PCR and Western blotting. Mos were analyzed by flow cytometry. A mouse colitis model was created with trinitrobenzene sulfonic acid. The results showed that mouse IECs produced IGF1, which could be up regulated by exposure to CpG-ODN (CpG-oligodeoxynueleotides) in the culture. Culture the CpG-ODN-primed IEC cells and Mos or exposure of Mos to IGF1 in the culture induced the Mos to express IL-10. The IGF1-primed Mos showed the immune suppressive effect on inhibiting the immune inflammation in the mouse colon. In conclusion, the IGF1-primed Mos are capable of suppressing immune inflammation in the intestine.

引用

页数：7

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