Low molecular weight heparin improves proteinuria in rats with L-NAME induced preeclampsia by decreasing the expression of nephrin, but not podocin

被引：12

作者：

Liu, Wanlu ^{[1
]}

Qiao, Fuyuan ^{[1
]}

Liu, Haiyi ^{[1
]}

Gong, Xun ^{[1
]}

Shi, Xinwei ^{[1
]}

Li, Yuqi ^{[1
]}

Wu, Yuanyuan ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Obstet & Gynecol, Wuhan 430000, Peoples R China

来源：

HYPERTENSION IN PREGNANCY | 2015年 / 34卷 / 01期

关键词：

Animal model; low-molecular-weight-heparin; nephrin; podocin; preeclampsia; KIDNEY; GENE; SECTIONS; WOMEN;

D O I：

10.3109/10641955.2014.951655

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: We investigated the relationship between proteinuria in L-NAME induced preeclampsia and the expression of nephrin and podocin, and the effect of low-molecular-weight-heparin (LMWH) on proteinuria in rats. Methods: We detected nephrin and podocin expression of kidneys of pregnant rats after L-NAME and after LMWH intervening pregnant rats. Results: Glomerular nephrin expression in L-NAME induced preeclampsia significantly decreased, but not podocin. Nephrin was relatively increased after LMWH intervention and this was accompanied by a decrease in proteinuria. Conclusion: We demonstrate that down-regulation of nephrin is involved in L-NAME induced proteinuria, and that LMWH reduces proteinuria by up-regulation of neprhin.

引用

收藏

页码：24 / 35

页数：12

相关论文

共 25 条

[1] Low molecular weight heparin reduces proteinuria and modulates glomerular TNF-α production in the early phase of adriamycin nephropathy [J].

Benchetrit, S ;

Golan, E ;

Podjarny, E ;

Green, J ;

Rashid, G ;

Bernheim, J ;

Hershkovitz, R ;

Bernheim, J .

NEPHRON, 2001, 87 (02) :155-160

[2] Proteinuria-lowering effect of heparin therapy in diabetic nephropathy without affecting the renin-angiotensin aldosterone system [J].

Benck, Urs ;

Haeckel, Sarah ;

Clorius, John H. ;

van der Woude, Fokko J. .

CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2007, 2 (01) :58-67

[3] Preeclamptic sera induce nephrin shedding from podocytes through endothelin-1 release by endothelial glomerular cells [J].

Collino, Federica ;

Bussolati, Benedetta ;

Gerbaudo, Elisa ;

Marozio, Luca ;

Pelissetto, Simona ;

Benedetto, Chiara ;

Camussi, Giovanni .

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2008, 294 (05) :F1185-F1194

[4] Inflammation in rat pregnancy inhibits spiral artery remodeling leading to fetal growth restriction and features of preeclampsia [J].

Cotechini, Tiziana ;

Komisarenko, Maria ;

Sperou, Arissa ;

Macdonald-Goodfellow, Shannyn ;

Adams, Michael A. ;

Graham, Charles H. .

JOURNAL OF EXPERIMENTAL MEDICINE, 2014, 211 (01) :165-179

[5] Low molecular weight heparin in the treatment of puromycin-induced nephrosis [J].

dos Santos, AMR ;

de Olveira, AV ;

Lemos, CCD ;

Mandarim-de-Lacerda, CA ;

Bregman, R .

PATHOLOGY RESEARCH AND PRACTICE, 2006, 202 (03) :157-163

[6] Inhibitory Effects of Robo2 on Nephrin: A Crosstalk between Positive and Negative Signals Regulating Podocyte Structure [J].

Fan, Xueping ;

Li, Qinggang ;

Pisarek-Horowitz, Anna ;

Rasouly, Hila Milo ;

Wang, Xiangling ;

Bonegio, Ramon G. ;

Wang, Hang ;

McLaughlin, Margaret ;

Mangos, Steve ;

Kalluri, Raghu ;

Holzman, Lawrence B. ;

Drummond, Iain A. ;

Brown, Dennis ;

Salant, David J. ;

Lu, Weining .

CELL REPORTS, 2012, 2 (01) :52-61

[7] Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors [J].

Feng, D ;

Nagy, JA ;

Brekken, RA ;

Pettersson, A ;

Manseau, EJ ;

Pyne, K ;

Mulligan, R ;

Thorpe, PE ;

Dvorak, HF ;

Dvorak, AM .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2000, 48 (04) :545-555

[8] Glomerular expression of nephrin and synaptopodin, but not podocin, is decreased in kidney sections from women with preeclampsia [J].

Garovic, Vesna D. ;

Wagner, Steven J. ;

Petrovic, Lydia M. ;

Gray, Catherine E. ;

Hall, Pauline ;

Sugimoto, Hikaru ;

Kalluri, Raghu ;

Grande, Joseph P. .

NEPHROLOGY DIALYSIS TRANSPLANTATION, 2007, 22 (04) :1136-1143

[9] Urinary podocyte excretion as a marker for preeclampsia [J].

Garovic, Vesna D. ;

Wagner, Steven J. ;

Turner, Stephen T. ;

Rosenthal, David W. ;

Watson, William J. ;

Brost, Brian C. ;

Rose, Carl H. ;

Gavrilova, Larisa ;

Craigo, Paula ;

Bailey, Kent R. ;

Achenbach, Johannes ;

Schiffer, Mario ;

Grande, Joseph P. .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2007, 196 (04) :320.e1-320.e7

[10] Interaction with podocin facilitates nephrin signaling [J].

Huber, TB ;

Köttgen, M ;

Schilling, B ;

Walz, G ;

Benzing, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (45) :41543-41546