Endoscopic Ultrasound-Guided Fine-Needle Aspiration With Liquid-Based Cytologic Preparation in the Diagnosis of Primary Pancreatic Lymphoma

被引：28

作者：

Rossi, Esther Diana ^{[1
]}

Larghi, Alberto ^{[2
]}

Verna, Elizabeth C. ^{[2
]}

Martini, Maurizio ^{[1
]}

Galasso, Domenico ^{[2
]}

Carnuccio, Antonella ^{[2
]}

Larocca, Luigi Maria ^{[1
]}

Costamagna, Guido ^{[2
]}

Fadda, Guido ^{[1
,2
]}

机构：

[1] Catholic Univ, Dept Pathol, Rome, Italy

[2] Catholic Univ, Digest Endoscopy Unit, Rome, Italy

来源：

PANCREAS | 2010年 / 39卷 / 08期

关键词：

EUS-FNA; primary pancreatic lymphoma; liquid-based cytologic preparation; THIN-LAYER CYTOLOGY; FLOW-CYTOMETRY; CONVENTIONAL PREPARATIONS; IMMUNOCYTOCHEMISTRY; EUS; THINPREP(R); NEOPLASMS; BIOPSIES; TUMORS; FNA;

D O I：

10.1097/MPA.0b013e3181dc694e

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Objectives: The diagnosis subtyping of lymphoma on specimens collected by endoscopic ultrasound fine-needle aspiration (EUS-FNA) can be extremely difficult. When a cytopathologist is available for the on-site evaluation, the diagnosis may be achieved by applying flow cytometric techniques. We describe our experience with immunocytochemistry (ICC) and molecular biology studies applied on EUS-FNA specimens processed with a liquid-based cytologic (LBC) preparation for the diagnosis of primary pancreatic lymphoma (PPL). Methods: Three patients with a pancreatic mass underwent EUS-FNA. The collected specimens were processed with the ThinPrep method for the cytologic diagnosis and eventual additional investigations. Results: A morphologic picture consistent with PPL was found on the LBC specimens of the 3 patients. Subsequent ICC and molecular biology studies for immunoglobulin heavy chain gene rearrangement established the diagnosis of pancreatic large B-cell non-Hodgkin lymphoma in 2 patients and a non-Hodgkin lymphoma with plasmoblastic/immunoblastic differentiation in the remaining one. Conclusions: An LBC preparation can be used to diagnose and subtype PPL by applying ICC and molecular biology techniques to specimens collected with EUS-FNA. This method can be an additional processing method for EUS-FNA specimens in centers where on-site cytopathologist expertise is not available.

引用

页码：1299 / 1302

页数：4

共 37 条

[1] Cytologic artifacts and pitfalls of thyroid fine-needle aspiration using ThinPrep® -: A comparative retrospective review [J].