Analysis of 11 tetrameric STRs in wild boars for forensic purposes

被引：22

作者：

Caratti, Stefano ^{[1
]}

Rossi, Luca ^{[2
]}

Sona, Bruno ^{[3
]}

Origlia, Silvia ^{[3
]}

Viara, Silvana ^{[3
]}

Martano, Giuseppe ^{[4
]}

Torre, Carlo ^{[1
]}

Robino, Carlo ^{[1
]}

机构：

[1] Univ Turin, Dept Anat Pharmacol & Legal Med, I-10126 Turin, Italy

[2] Univ Turin, Dept Anim Prod Epidemiol & Ecol, I-10095 Grugliasco, TO, Italy

[3] Serv Vet SC Sanita Anim, ASL CN1 Reg Piemonte, I-12038 Savigliano, CN, Italy

[4] Serv Vet ASL TO3, Area Sanita Anim, I-10064 Pinerolo, TO, Italy

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2010年 / 4卷 / 05期

关键词：

Sus scrofa; Short tandem repeat; Forensic genetics; SUS-SCROFA; MICROSATELLITE ANALYSIS; POPULATIONS; SOFTWARE; SWINE;

D O I：

10.1016/j.fsigen.2010.07.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

STR profiling of animal species has a wide range of applications, including forensic identification, wildlife preservation, veterinary public health protection and food safety. We tested the efficacy of a multiplex PCR-based assay including 11 porcine-specific tetrameric STRs in a population sample of wild boars (n = 142) originating from Piedmont (North West Italy). Multiple deviations from Hardy-Weinberg expectations were observed, mostly due to a reduction in observed heterozygosity indicative of a high degree of inbreeding. A value of theta of 0.046 and an inbreeding coefficient of 0.089 were estimated. Combined power of discrimination and probability of exclusion values for the STR panel were 0.9999999999996 and 0.99989. In order to test the suitability of the method for meat traceability purposes, a domestic pig reference sample (n = 412), consisting of commercial lines commonly used in the meat production process, was also typed. A Bayesian cluster analysis carried out using the observed genotypes, showed a percentage of correct subspecies assignment of individual samples of 0.974 for wild boars and 0.991 for pigs, thus demonstrating the usefulness of the multiplex STR-typing system for discrimination purposes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：339 / 342

页数：4

共 18 条

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