Intergenotypic chimeric hepatitis E viruses (HEVs) with the genotype 4 human HEV capsid gene in the backbone of genotype 3 swine HEV are infectious in pigs

被引：39

作者：

Feagins, Alicia R. ^{[1
]}

Cordoba, Laura ^{[1
]}

Sanford, Brent J. ^{[1
]}

Dryman, Barbara A. ^{[1
]}

Huang, Yao-Wei ^{[1
]}

LeRoith, Tanya ^{[1
]}

Emerson, Suzanne U. ^{[2
]}

Meng, Xiang-Jin ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Coll Vet Med, Dept Biomed Sci & Pathobiol, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA

[2] NIAID, Mol Hepatitis Sect, Infect Dis Lab, NIH, Bethesda, MD 20892 USA

来源：

VIRUS RESEARCH | 2011年 / 156卷 / 1-2期

基金：

美国国家卫生研究院;

关键词：

Hepatitis E virus (HEV); Chimeric viruses; Cross-species infection; Pigs; Host range; CROSS-SPECIES INFECTION; CIS-REACTIVE ELEMENT; ORF3; PROTEIN; RECEPTOR-BINDING; VIRAL-RNA; IN-VITRO; CELLS; REPLICATION; HEPATOMA; RELEASE;

D O I：

10.1016/j.virusres.2010.12.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Genotypes 1 and 2 hepatitis E virus (HEV) infect only humans whereas genotypes 3 and 4 HEV infect both humans and pigs. To evaluate the mechanism of cross-species HEV infection between humans and swine, in this study we constructed five intergenotypic chimeric viruses and tested for their infectivity in vitro and in pigs. We demonstrated that chimeric viruses containing the ORF2 capsid gene either alone or in combination with its adjacent 5' junction region OR) and 3' noncoding region (NCR) from a genotype 4 human HEV in the backbone of a genotype 3 swine HEV are replication-competent in Huh7 cells and infectious in HepG2/C3A cells and in pigs, and thus supporting the hypothesis that genotypes 3 and 4 human HEV are of swine origin. However, chimeric viruses containing the JR+ ORF2 + 3' NCR of genotypes :3 or 4 HEV in the backbone of genotype 1 human HEV failed to infect pigs, suggesting that other genomic regions such as 5' NCR and ORF1 may also be involved in HEV cross-species infection. The results from :his study provide the first experimental evidence of the exchangeability of the capsid gene between genotype 3 swine HEV and genotype 4 human HEV, and have important implications for understanding the mechanism of HEV cross-species infection. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：141 / 146

页数：6

共 34 条

[21] Experimental infection of pigs with the newly identified swine hepatitis E virus (swine HEV), but not with human strains of HEV [J].